两种人工辅助孵化方法在IVF-ET中的比较性研究  

A comparative study of two assisted-hatchingMethods in IVF transfer.

作　　者：张若鹏[1] 邵华[1] 张丽蓉[1] 刀承兰[1] 朱莉[1] 

机构地区：[1]大理医学院附属医院生殖助孕中心,云南大理671000

出　　处：《中国优生与遗传杂志》2005年第2期105-106,共2页Chinese Journal of Birth Health & Heredity

基　　金：云南省教育厅科研基金 (NO 0 3Y63 2C)资助

摘　　要：目的　探讨两种辅助孵化方法对体外受精 -胚胎移植 (IVF -ET) )临床结果的影响 ,以便选择较好方法。方法　在体外受精后胚胎培养的第 2d ,用Protease消化和机械磨擦两种孵化方式分别对年龄超过 32岁的各 30例妇女的移植胚胎进行人工孵化。结果　采用Protease 30例辅助孵化 ,妊娠 11例 ,妊娠率 36 .6 % ;采用机械辅助孵化 30例 ,妊娠 8例 ,妊娠率 2 6 .7% ,两者有显著性差异 (P <0 .0 5 )。结论　Protease孵化法与机械磨擦孵化法相比具有方法简单、操作时间短、成本低、妊娠率高 ,有重要应用推广价值。Objective:this study is to determine the offect of two assisted-hatching methods on the clinical results of in vitro fertilization(IVF). Methods: In the second day of embryo cultivation,assisted hatching is made to the transferred enbryos of thirty 32-year-old women respectively by digestion with Protease and by mechanical partial zone dissection(PZD). Results: Assisted hatching by the method of digestion with Protease helps 11 of the 30 tested women to be pregnant with the pregnant rate being 36.6%; assisted hatching by the method of mechanical PZD helps 8 of the 30 tested women to be pregnant with the pregnant rate being 26.7%. So there is a great difference(P<0.05)in pregnant rates by the two methods. Conclusion: compared with PZD, digestion with Protease is simpler with shorter time, lower cost and higher pregnant rate, so it is of higher practical value.

关 键 词：人工辅助孵化 体外受精 胚胎移植 

分 类 号：R711.6[医药卫生—妇产科学]