烟草曲叶双生病毒的研究——Ⅰ.广西分离物的生物学特性和血清学反应  

STUDIES ON TOBACCO LEAF CURL VIRUS(TLCV)IN GUANGXI PROVINCE OF CHINA——Ⅰ.BIOLOGICAL PROPERTIES AND SEROLOGICAL RELATIONSHIP WITH AFRICAN CASSAVA MOSAIC VIRUS(ACMV)

作　　者：蔡健和[1] 王小凤[2] 黄福新[1] 林明生[1] 王文慧[2] 田波[2] 

机构地区：[1]广西农业科学院植物保护研究所,南宁530007 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1993年第3期166-169,共4页Acta Microbiologica Sinica

摘　　要：烟草曲叶病已在广西九个产烟县(市)发生危害,一般为零星发生,局部地区平均发病率高达50%以上,造成严重经济损失。试验结果表明,此病仅可通过嫁接和烟粉虱(Bemisiatabaci)传染,而汁液摩擦、种子和中国菟丝子(Cuscuta chinesis)不传病。除普通烟(Nicotianatabacum)外,还可侵染枯斑三生烟(N.tabacum var.xanthi N)、番茄(Lycopersicon esculentum)和蔓陀罗(Datura stramonium)等鉴别寄主,产生典型的曲叶症状。ELISA 检测结果表明,病烟叶提取液与非洲木薯花叶病毒(ACMV)的两个单抗(SCR 18和 SCR 23)呈阳性反应,而与另外两个单抗(ACMV SCR 11和 ICMV(印度木薯花叶病毒)SCR 52)呈阴性反应,用ACMV 单抗 SCR 18免疫吸附法制片,在电镜下观察到球状成对或单个的病毒颗粒,单颗粒直径为18nm,成对颗粒大小为15—18×25—30nm。Tobacco leaf curl disease was found in nine counties of Guangxi province,in general,only a few tobacco plant was infected,but in some places,the disease incidence reachedmore than 50 percent,and caused great damage to the production of tobacco.It has beenshown that this disease could be transmitted only by grafting and Bemisia tabaci,and notby seed,Cuscuta chinensis and mechanical inoculation.Besides Nicotiana tabacum,it couldinfect Lyconpersicon esculentum,Datura stramonium and N.tabacum var.xanthi N andcaused typical leaf curl symptom.ELISA test show that the leaf extract from diseasedtobacco reacted positively with two Mabs(SCR 18 and SCR 23)of ACMV,but negativelywith ACMV Mab SCR 11 and I CMV Mab SCR 52.I SEM showed that single or pairedspherical virus particles could be trapped by Mab SCR 18 of ACMV,the single virus particleswas 18nm in diameter,and the size of paired virus particles was 15—18×25—30nm.It isclear from these data that the pathogen which induced tobacco leaf curl disease belong geminiviruses.Further study is in progress.

关 键 词：血清学 烟草曲叶病毒 双生病毒 

分 类 号：S432.41[农业科学—植物病理学]