一株A型肉毒梭状芽孢杆菌的分离与鉴定  

THE ISOLATION AND IDENTIFICATION OF A CLOSTRIDIUM BOTULINUM SEROTYPE A STRAIN

作　　者：刘世贵[1] 伍铁桥[1] 杨志荣[1] 袁涛 

机构地区：[1]四川大学生物工程研究所

出　　处：《微生物学报》1993年第4期280-284,共5页Acta Microbiologica Sinica

基　　金：国家教育委员会资助课题

摘　　要：从四川成都、重庆、若尔盖,新疆乌鲁木齐,宁夏固原共采集土样319份进行肉毒梭状芽孢杆菌的分离。在若尔盖土样中分离到一株厌氧芽孢杆菌,按《伯杰细菌鉴定手册》(第八版)的方法进行了生物学测定、生理生化试验,并测定了其 DNA 中 G+C mol 为24.9%,鉴定为肉毒梭状芽孢杆菌,经血清学试验进一步鉴定为 A 型肉毒梭菌,编号为 As-3。319 soil specimens were collected from different places of China for isolating Clostridium botulinum.A strain of Clostridium botulinum was isolated from a culture of soil specimens in Ruoergai of Sichuan Province,the strain was called As-3. The As-3 was identified as Clostridium botulinum serotype A according to its biological properties,biochemical serological and toxicological characteristics and DNA determination. Its DNA G+C mol is 24.9%.The toxin produced by As-3 strain can only be neutralized by type A antiserum.

关 键 词：分离 肉毒芽孢杆菌 梭状芽孢杆菌 

分 类 号：Q939.11[生物学—微生物学]