氧代赖氨酸抗真菌作用机制的初步研究  

PRELIMINARY EXPERIMENTS ON THE MODE OF ACTION OF OXALYSINE ON CANDIDA ALBICANS and C.PARAPSILOSIS

作　　者：张鸿龙 顾秀玉 王蕖 J.M.Pecker 

机构地区：[1]中国科学院上海药物研究所,上海200031 [2]美国田纳西大学微生物系

出　　处：《微生物学报》1993年第6期411-417,共7页Acta Microbiologica Sinica

摘　　要：氧代赖氨酸对致病真菌类秃发念珠菌的ＭＩＣ为０．８－３．１μｇ／ｍｌ，而两性霉素Ｂ为１．６－３．１μｇ／ｍｌ．氧代赖氨酸高于赖氨酸１０倍浓度，无明显抑制白含珠菌对Ｌｙｓｉｎｅ的吸收。Ｏｘａｌｙｓｉｎｅ０．４ｍｍｏｌ／Ｌ对白色念珠菌细胞膜透性，如胞内物于ＵＶ２６０ｎｍ下的吸收物质外泄无明显影响。放射性前体物渗入试验结果指出，Ｏｘａｌｙｓｉｎｅ０．４ｍｍｏｌ／Ｌ弱抑制（＾１４Ｃ）－Ｍｅｔｈｉｏｎｉｎ和（Oxalysine,NH_2—CH_2—O—CH_2——-COOH is a novel anti-fungal antibioticisolated from Streptomyces roseoviridofuscus n.sp..MIC values 0.8—3.lμg/mlsuggested a good activity of oxalysine against Candida parapsilosis when compared toAmphotericin-B and 5-Fluorocytosine.The MIC for Trichophyton rubrum andMicrosporeum gypseum range from 1.56—3.13μg/ml.Transportation of radioactivity labelled(~3H)-Lysine compete with oxalysine wasused to characterize Lysine permease in Candida albicans.The results showed thatoxalysine 10-folds did not reduce the rate of(~3H)-Lysine uptake.This showed lowaffinity of lysine permcase to oxalysine in Candida albicans.When the leakage of U V260 nm-absorbing materials wits used as the evidence of permeability of the cellmembrane.Oxalysine was not observed to cause leakage of cellular constituents.Theresult showed that the action of oxalysine is not on the cell membrane of the micro-organism.The incorporations of(^(14)C)-Methione and (^(14)C)-Adenine were used as amethod of measuring the antimicrobial action of the drug oxalysine is on protein and/ornucleic acid synthesis.It was found that oxalysine 0.4 mmol/L did not significantlyinhibit their incorporations into protein and/or DNA,but strongly inhibit theincorporation of(^(14)C)-Adenine into RNA.

关 键 词：氧代赖氨酸 白色念珠菌 真菌 药物 

分 类 号：R915[医药卫生—微生物与生化药学]