冷冻方法和冷冻保护剂对人精子顶体酶活性影响  

Effects of different cryopresevation methods and medium on acrosin activities of human spermatozoa

作　　者：刘睿智[1] 董乃屹 许宗革[1] 孙妍[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室,吉林长春130021 [2]吉林省计划生育研究所,吉林长春130000

出　　处：《吉林医学》2005年第2期144-145,共2页Jilin Medical Journal

基　　金：吉林省计划生育委员会资助项目(2003)

摘　　要：目的:了解冷冻方法和冷冻保护剂对人精子顶体酶活性影响。方法:对精液标本进行速冻和缓慢冷冻保存,应用分光光度比色法测定精子顶体酶活性。结果:速缓冷冻法对精子顶体酶活性无显著性影响(P>0.05)。甘油-卵黄-柠檬酸钠(GYC)组顶体酶活性明显高于单一甘油组。结论:冷冻速度对人精子顶体酶活性没有影响(P<0.05),应用GYC作为CPM可使人精子顶体酶活性保持很高活性。Objective To study the effect of different cryporotective mediums (CPMs) and freezing methods on sperm acrosin activity. Method The sperm acrosin activities were determined by spectrophotometer. Results The acrosin activities of frozen-thawed human semen by slow and quick freezing-storing methods were not significantly different(P>0.05). The acrosin activities in GYC group are higher than those in glycerol group(P<0.05). Conclusion GYC has better preservation effect on human sperm than glycerol group.

关 键 词：顶体酶 冷冻保护剂 精子 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]