利用整合诱变技术进行棕色固氮菌nifH^-突变株的构建  

CONSTRUCTION OF AZOTOBACTER VINELANDII NIFHMUTANTS BY LNTEGRATION MUTAGENESIS

作　　者：吕群燕[1] 段俊英[2] 戴祥鹏 刘纯强[3] 

机构地区：[1]清华大学化工系生物化工与食品加工室,北京100084 [2]中科院沈阳应用生态所,110015 [3]山东大学微生物研究所,250010

出　　处：《微生物学杂志》1993年第2期1-3,12,共4页Journal of Microbiology

摘　　要：用含有克氏肺炎杆菌(Klebsiella Pneumoniae)nifH基因的质粒P^(pC12101)对棕色固氮菌(Azotobacter vinelandii)301进行了定点整合诱变,得到了29株抗氨苄青霉素的菌株。进一步的乙炔还原活性测定表明,其中可能含有12株第一固氮酶nifH基因突变的菌株,8株第二固氮酶nifH基因突变的菌株。Site directed integration mutagenesis using a plasmid which contains a nifH. gene from Klebsiella Pneumonial results in 29 ampicillin-resistant strains. Further detection of C_2H_2: reduce activity indicates that 12 strains of them may contain mutageneses in the nifH: gene of the first nitrogenase, 8 strains of them may contain mutageneses in the nifH gene region of second nitrogenase.

关 键 词：整合诱变 棕色固氮菌 突变株 

分 类 号：Q939.113[生物学—微生物学]