虎杖水煎液对体外培养感染柯萨奇B_3病毒大鼠心肌细胞保护作用的实验研究  

EXPERIMENTAL STUDY ON PROTECTIVE EFFECTS OF POLYGONUM CUSPIDATUM WATEREXACT ON RATS' CARDIAC MUSCLE CELL INFECTED BY COXSACKIE VIRUS B_3 IN VITRO

作　　者：申成华[1] 郑善子[1] 崔春权[1] 李太一 

机构地区：[1]延边大学医学院寄生虫学教研室,延吉1330001 [2]吉林省敦化市太一口腔诊所,敦化133700

出　　处：《中国中医药科技》2005年第1期34-35,共2页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的 :探讨虎杖水煎液对病毒性心肌炎的保护作用。方法 :实验取新生SD大鼠心室肌制备搏动心肌细胞 ,培养 4 8h后添加 10 3 TCID50 的柯萨奇B3 病毒作为实验模型。实验分为 4个组进行 ,即正常对照组、病毒对照组、阳性对照组 (干扰素 )组、虎杖水煎液组。结果 :虎杖水煎液明显提高细胞搏动百分比 ;明显增加细胞内糖原颗粒数目 ,增强琥珀酸脱氢酶活性 ,降低乳酸脱氢酶活性。结论 :虎杖水煎液对感染柯萨奇B3 病毒SD大鼠心肌细胞具有明显的保护作用。Objective:To discuss the protective effect of Polygonum cuspidatum water exact on viral myocarditis.Methods:The beating myocardial cells obtained from the ventricular myocardium of newborn Sparaque-Dawley rats and incubated for 48 hours and inoculated with 10 3 TCID50 of Coxsackie virus B 3 as experimental model of viral myocarditis.All rats were randomly divided into four groups:normal group,control group,positive interferon treated group and Polygonum cuspidatum water exact group.Results:Polygonum cuspidatum water exact increased percent of cell beating,obviously increased glucogen granule in myocardial cells,enanced the activity of SDH,reduced the activity of LDH.Conclusion:Polygonum cuspidatum water exact may protect SD rat myocardial cells from infecting coxackie virus B 3.

关 键 词：水煎液 虎杖 柯萨奇B3病毒 保护作用 心肌细胞 感染 对照组 TCID50 琥珀酸脱氢酶活性 乳酸脱氢酶活性 

分 类 号：R285[医药卫生—中药学]