几种荚膜红细菌变异体的光合制氢研究  

Research on Photosynthetic Hydrogen Production of Several Rhodobacter Capsulatus Mutants

作　　者：何德良[1] 周舟[1] 张小华[1] 陈四海[1] 

机构地区：[1]湖南大学化学化工学院,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2005年第1期73-77,共5页Journal of Hunan University:Natural Sciences

基　　金：湖南省自然科学基金资助项目(04JJ3012);化学生物传感与计量学国家重点实验室开放基金(200403)

摘　　要：研究了几种荚膜红细菌变异体(IR1,IR3,IR4,JP91)在DL-乳酸盐和L-谷氨酸盐分别为碳源与氮源时的光合成生长与光合制氢过程.通过测定培养液在660nm下吸光度的变化,实现了对不同荚膜红细菌变异体光合生长过程的监测,并讨论了这些变异体的生长特性.所得氢气采用排水法收集,测量了几种荚膜红细菌变异体光合产氢的平均速率、光合产氢量和底物转化效率,并与荚膜红细菌的野生型B10进行了对比.结果表明,它们产氢能力从强到弱的顺序依次为:IR3,JP91,IR4,B10和IR1.将所产生的氢气直接供给一个小型PEM燃料电池使用的实验也证明了它们的产氢能力的差异,其中,变异体IR3所产生的电流最持久.The photosynthetic growth and hydrogen production of Rhodobacter Capsulatus mutants (IR1,IR3,IR4,JP91), grown in a culture with 30 mmol L^(-1) DL-Lactate and 5 mmol L^(-1) L-Glutamate as the carbon and nitrogen source, were investigated. Their growth were measured by monitoring the increase in absorbance at 660 nm. The hydrogen produced by these strains was gathered in the drainage method. In this way, the hydrogen yield and substrate conversion efficiency of Rhodobacter Capsulatus mutants were obtained and compared with those of the wild-type strain B10. It was found that the order of their hydrogen production capabilities was IR3,JP91,IR4,B10,IR1. The hydrogen was raw used as feed for a small PEM fuel cell system and the difference of their hydrogen production capability was proved. Among them, IR3 produced the most durable electric current. 

关 键 词：荚膜红细菌 氢气 产氢能力 底物转化效率 

分 类 号：Q935[生物学—微生物学]