α-石英对离体巨噬细胞内游离Ca^(2+)的影响  

EFFECTS OF a-QUARTZ ON THE CONCENTRATION OF CYTOPLASMIC FREE CALCIUM ION OF THE MACROPHAGE IN VITRO

作　　者：温和瑞[1] 王金晞[1] 凌静[1] 张宇[1] 杨震[1] 陈荣三[1] 张祖暄[2] 

机构地区：[1]南京大学配位化学研究所配位化学国家重点实验室,南京210008 [2]南京大学医学院,南京210008

出　　处：《无机化学学报》1993年第3期266-270,共5页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助课题

摘　　要：本文用荧光试剂Fura-2/AM和AR-CM-MlC阳离子测定系统研究了α-石英对离体肺泡巨噬细胞内游离Ca^(2+)的影响.结果表明:在含Ca^(2+)介质中,α-石英对巨噬细胞的毒性作用引起胞浆游离Ca^(2+)浓度的升高,α-石英剂量越大或作用时间增长,胞浆游离Ca^(2+)浓度升高越大,这种效应只能部分地被Ca^(2+)通道阻断剂异搏定所阻断.但在无Ca^(2+)介质中未观察到细胞胞浆游离Ca^(2+)浓度升高的现象.In this paper, with fluorescence solvent Fura-2/AM and AR-CM cation measurement system to study the effects of α-quartz on the concentration of cytoplasmic free Ca(2+) of the macrophage in vitro. The results are mainly as follows: In the Ca(2+)- medium, the poisonous function of α-quartz to alveolar macrophage leads to the increase of the cytoplasmic free Ca2+ concentration. The higher the concentration of the a-quartz dust suspension, or the longer the time of action, the greater the increase of the concentration of cytoplasmic free Ca(2+) However, Ca(2+) channel blocker verapamil can partly inhibit the increase of the concentration of cytoplasmic free Ca(2+). But in the absence of Ca(2+), the concentration of cytoplasmic free Ca(2+) doesn't increase.

关 键 词：巨噬细胞 石英 游离 钙离子 

分 类 号：R135.2[医药卫生—劳动卫生]