大豆遗传图谱的构建和若干农艺性状的QTL定位分析  

Construction of Genetic Map and QTL Analysis for Some Agronomic Traits in Soybean

作　　者：杨喆[1] 关荣霞[1] 王跃强[2] 刘章雄[1] 常汝镇[1] 王曙明[2] 邱丽娟[1] 

机构地区：[1]中国农业科学院作物品种资源研究所/农业部作物种质资源与生物技术重点开放实验室 [2]吉林省农业科学院大豆研究所,公主岭136100

出　　处：《植物遗传资源学报》2004年第4期309-314,323,共7页Journal of Plant Genetic Resources

基　　金：国家科技攻关项目 (2 0 0 1BA5 11B0 6);国家"863"计划资助项目 (2 0 0 3AA2 0 70 60 )

摘　　要：大豆许多重要农艺性状都是由微效多基因控制的数量性状 ,对这些数量性状进行QTL定位是大豆数量性状遗传研究领域的一个重要内容。本研究利用栽培大豆科新 3号为父本、中黄 2 0为母本杂交得到含 192个单株的F2 分离群体 ,构建了含 12 2个SSR标记、覆盖 1719 6cM、由 33个连锁群组成的连锁遗传图谱。利用复合区间作图法 ,对该群体的株高、主茎节数、单株粒重和蛋白质含量等农艺性状的调查数据进行QTL分析 ,共找到两个株高QTL ,贡献率分别为 9 15 %和 6 0 8% ;两个主茎节数QTL ,贡献率分别为 10 1%和 8 6 % ;一个蛋白质含量QTL ,贡献率为 9 8% ;一个单株粒重QTL ,贡献率为11 4 %。通过遗传作图共找到与所定位的 4个农艺性状QTL连锁的 6个SSR标记 ,这些标记可以应用于大豆种质资源的分子标记辅助选择 ,从而为大豆分子标记辅助育种提供理论依据。Many important agronomic traits of soybean are quantitative traits, which are controlled by polygenes. Tagging QTL for these quantitative traits is a very important field in soybean quantitative genetics research. Kexin 3 and Zhonghuang 20 are releasing cultivars in Huanghuaixia ecological district with excellent quality traits. A cross of Kexin 3 and Zhonghuang 20 was made, then selfed to construct F 2 population which has 192 plants. A genetic linkage group of soybean genome was constructed, which consisted 33 linkage groups with 122 SSR markers covering 1719 6 cM. The composite interval mapping was used to identify quantitative trait loci (QTL) associated with plant height, nod number of main stem and protein content. Two QTL were found for plant height with the phenotypic variance explained of 9 15% and 6 08% respectively; two for nods number of main stem with the phenofypic variance explained of 10 1% and 8 6% respectively; one for protein content with the phenotypic variance explained of 9 8%. Six markers linked to the QTL for four agronomic traits were found and can be used in molecular marker assisted selection in soybean.

关 键 词：QTL定位 大豆 农艺性状 单株 遗传图谱 株粒重 主茎 连锁群 研究利用 豆科 

分 类 号：S565.1[农业科学—作物学]