[Cu(dien)Cl]^+与核苷酸作用的~1H、^(31)P NMR研究  

~1H and ^(31)P NMR STUDIES OF THE INTERACTION OF [Cu(dien)Cl]^+ WITH NUCLEOTIDES

作　　者：赵刚[1] 吴仲岿[1] 管荫桐 唐雯霞[1] 

机构地区：[1]南京大学配位化学研究所,配位化学国家重点实验室,南京210008

出　　处：《无机化学学报》1993年第1期7-11,共5页Chinese Journal of Inorganic Chemistry

摘　　要：本文用~1H-NMR和^(31)P-NMR谱研究了[Cu(dien)Cl]^+与5＇-AMP、5＇-GMP和5＇-CMP在pD=6.00条件下的共价键合作用.氢核磁共振谱研究表明[Cu(dien)]^(2+)与5＇-AMP可以在N-7和N-1位上键合,但N-7是最有利的键合位置.配离子与5＇-GMP、5＇-CMP、分别键合在N-7和N-3位置上.^(31)P核磁共振谱研究结果则表明[Cu(dien)]^(2+)还可以与单核苷酸上的磷酸酯根键合.并根据显著加宽质子峰及^(31)P峰的最低[Cu(dien)Cl]^+浓度,比较了[Cu(dien)]^(2+)对三种核苷酸的碱基与磷酸酯根的亲力.The interaction of [Cu(dien)Cl]+ with 5'-AMP, 5'-GMP, and 5'-CMPat pD = 6.0 have been studied by 1H and 31P-NMR spectroscopy. The broadening effect of [Cu(dien)Cl]+ on the 1H peaks of nucleotides reveal [Cu(dien)]2+ binding to N-7 of 5'-GMP and N-3 of 5'-CMP respectively, and binding 5-AMP at N-7 and N-l simultaneously, but N-7 is the mere preferential position. The 31P-NMR spectra indicate that [Cu(dien)]2+ can also bind with phosphate of mentioned nucleotides. Comparing the lowest concentration of [Cu(dien)Cl]+, at which the apparent broadening of proton and 31P peaks occur, the affinity of [Cu(dien)]2+ to the N atoms on bases and the phosphate was discussed.

关 键 词：铜 核苷酸 NMR 

分 类 号：O614.121[理学—无机化学]