Improvement of polyploidy induction in Eriocheir sinensis  

作　　者：CUIZhaoxia XIANGJianhai ZHOULinghua CAINaner SONGLinsheng 

机构地区：[1]EMBL,InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China

出　　处：《Acta Oceanologica Sinica》2004年第4期725-732,共8页海洋学报（英文版）

基　　金：This project was supported by the IFS under contract No.A3225-1;the National Key Fundamental Research Program(973)of China under contract No.G1999012008.

摘　　要：An incubating temperature of 15 C is used to induce triploidy in Eriocheir sinensis through inhibition of the releaseof polar body II, and that of 18 C to induce tetraploidy through inhibition of the first cleavage. Flow cytometry isused to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, thehighest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCl is 49.1%, 51.7% and 77.5%,respectively. In the KCl treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rateobserved in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula bycytochaslasin B, 6-DMAP and KCl is 50.3%, 54.9% and 79.8% respectively. In the KCl treatment of pregnant crabswith the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitroculture is overcome. Triploid zoea Eriocheir sinensis has been developed for the first time. The induction rate oftetraploid zoea has also been greatly improved.An incubating temperature of 15 C is used to induce triploidy in Eriocheir sinensis through inhibition of the releaseof polar body II, and that of 18 C to induce tetraploidy through inhibition of the first cleavage. Flow cytometry isused to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, thehighest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCl is 49.1%, 51.7% and 77.5%,respectively. In the KCl treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rateobserved in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula bycytochaslasin B, 6-DMAP and KCl is 50.3%, 54.9% and 79.8% respectively. In the KCl treatment of pregnant crabswith the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitroculture is overcome. Triploid zoea Eriocheir sinensis has been developed for the first time. The induction rate oftetraploid zoea has also been greatly improved.

关 键 词：Eriocheir sinensis TRIPLOID TETRAPLOID ZOEA 

分 类 号：S932.52[农业科学—渔业资源] Q78[农业科学—水产科学]