地衣芽孢杆菌海水生化需氧量传感器研究  

BOD Biosensor for Seawater Using Bacillus Licheni Formis

作　　者：马莉[1] 崔建升[1] 王晓辉[1] 刘俊稚[1] 张洁[1] 

机构地区：[1]河北科技大学环境科学与工程学院,河北石家庄050018

出　　处：《河北科技大学学报》2004年第4期83-85,共3页Journal of Hebei University of Science and Technology

基　　金：国家"八六三"计划课题(2001AA635100)

摘　　要：用经海水驯化的地衣芽孢杆菌作响应菌株,采用夹层膜法制作固定化微生物膜,以氧电极为换能器制成BOD生物传感器;以空白海水为底液,传感器在BOD质量浓度为0～27mg/L范围内线性相关系数r=0.99978,对BOD质量浓度为4.31mg/L的GGA溶液连续平行测定7次,相对标准偏差为2.56%。Based on oxygen electrode,a BOD biosensor for seawater has been fabricated with immobilized cells using double-layered membrane method. The biological sensing material is immobilized Bacillus licheniformis tamed in saline water. With blank seawater as substrate, characteristics of the biosensor are tested. The biosensor has a linear response of 0 to 27 mg/L BOD and the linearity is perfect(r=0.999 78). The standard deviation of continuous measurements (n=7) is 2.56%. The sensor shows stability within 40 days without any decrease in signal.

关 键 词：BOD 生化需氧量 质量浓度 固定化微生物 海水驯化 膜法 制成 地衣芽孢杆菌 水生 菌株 

分 类 号：X832[环境科学与工程—环境工程] S965[农业科学—水产养殖]