缺氧对世居高原藏族人脐静脉内皮细胞ET和NO水平的影响  

EFFECTS OF HYPOXIA ON ET AND NO LEVELS IN CULTURED UMBILICAL VENOUS ENDOTHELIAL CELLS OF NATIVE TIBETAN

作　　者：高文祥[1] 高钰琪[1] 李素芝[2] 张国斌[1] 宋玲[1] 孙秉庸[1] 史景泉[3] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学研究室,全军高原生理与高原病研究重点实验室,重庆400038 [2]西藏军区总医院,西藏拉萨850001 [3]第三军医大学西南医院病理科,重庆400038

出　　处：《中国应用生理学杂志》2005年第1期1-4,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金重大项目 (3 0 3 93 13 0 )

摘　　要：目的 :观察世居高原藏族人静脉血ET和NO含量和缺氧对培养脐静脉内皮细胞ET和NO水平的影响 ,以期从胎儿生长发育的角度探讨人类高原适应的机制。方法 :分别以放射免疫分析法和Greiss法测定世居高原藏族、移居高原汉族和平原汉族人静脉血ET和NO含量。体外培养世居高原藏族和移居高原汉族新生儿UVECs,分为 4组 :①世居高原藏族人UVECs常氧组 (TC) ;②世居高原藏族人UVECs缺氧组 (TH) ;③移居高原汉族人U VECs常氧组 (HC) ;④移居高原汉族人UVECs缺氧组 (HH) ,收集培养上清液测定ET和NO含量。结果 :世居高原藏族人静脉血NO水平显著高于移居高原汉族人 ,而ET水平显著低于移居高原汉族人。体外低氧 (0 .5 %O2 )条件培养 12h和 2 4h时 ,TH组ET浓度显著低于HH组 ,而HH组与TH组和HC组与TC组相应时间点之间的NO浓度差异无显著性意义。结论 :缺氧时世居高原藏族人UVECsET分泌增高的程度较低 ,可能有助于保持相对低的血管张力 ,有利于胎儿血液供应。Aim: To assay ET and NO in venous blood of native Tibetan and to investigate the effects of hypoxia on ET and NO levels in cultured umbilical venous endothelial cells of native Tibetan. Methods: ET and NO in venous blood of native Tibetan, immigrant Han and lowland Han were assayed. Umbilical venous endothelial cells(UVECs) from native Tibetan and immigrant Han newborns were cultured and divided into 4 groups: ① Native Tibetan control group(TC), ② Native Tibetan hypoxic group(TH), ③ Immigrant Han control group(HC), ④ Immigrant Han hypoxic group(HH). Supernatant was collected and ET and NO were detected. Results: Venous blood NO was significantly higher in native Tibetan than in immigrant Han, while ET lower in native Tibetan than in immigrant Han. ET excretion from UVECs was elevated while NO decreased in both Tibetan and Han groups after exposed to hypoxia. On time-points 12 h and 24 h, ET was significantly lower in TH than in HH, while concentration of NO showed no difference in TH and HH. Conclusion: ET released by UVECs was higher in Han than in Tibetan after 12 h and 24 h hypoxic exposure, which may be in favor of lower vascular resistance and better fetal blood supply in Tibetan, and thus plays a role in the mechanisms of less intrauterine growth restriction(IUGR) throughout pregnancy and heavier birth weight of Tibetan newborns.

关 键 词：高原 藏族 缺氧 一氧化氮 内皮素 

分 类 号：R338[医药卫生—人体生理学]