pEGFP-N1质粒转染乳鼠心肌细胞的分布及效率  

DISTRIBUTION AND EFFICIENCY OF pEGFP-N1 PLASMID AFTER ITS TRANSFECTION IN CARDIOMYOCYTES

作　　者：冯红[1] 徐文琳[1] 战锐[1] 钱令嘉[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》2005年第1期112-114,i004,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目 (3 0 3 70 5 86)

摘　　要：目的 :研究 pEGFP N1质粒转染心肌细胞的分布及效率。 方法 :培养乳鼠心肌细胞 ,根据乳鼠心肌细胞的不同生长时间 (1～ 3d)进行 pEGFP N1质粒转染心肌细胞的实验研究。 结果 :乳鼠心肌细胞生长 1d时 ,pEGFP N1质粒转染心肌细胞的效率显著高于乳鼠心肌细胞生长 2d、3d时 ;pEGFP N1质粒转染心肌细胞后EGFP均匀地充满胞浆和胞核。结论 :pEGFP N1质粒转染乳鼠心肌细胞的效率与心肌细胞的生长期有关 ;EGFP在心肌细胞中均匀分布于胞浆和胞核。Aim: To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes. Methods: The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied. Results: The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus. Conclusion: The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.

关 键 词：pEGFP-N1质粒 转染 心肌细胞 分布 转染效率 

分 类 号：R338.2[医药卫生—人体生理学]