糖尿病视网膜病变的二级康复干预:人血管生成素1的克隆和分析  

Secondary rehabilitation preventive intervention for diabetic retinopathy: cloning and sequence analysis of human angiopoietin -1

作　　者：马腾[1] 刘学政[1] 刘丽波[2] 黄波[1] 

机构地区：[1]锦州医学院解剖教研室,辽宁省锦州市121001 [2]中国医科大学神经生物学教研室,辽宁省沈阳市110000

出　　处：《中国临床康复》2005年第7期50-51,i002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁省自然科学基金资助项目(20022152);辽宁省科技计划资助项目(2003225007)~~

摘　　要：目的:从人的胎盘中克隆血管生成素(angiopoietin-1,Ang-1)基因,并分析其结构特征。方法:提取人胎盘组织总RNA,用反转录聚合酶链式反应(RT-PCR)技术扩增出Ang-1基因片段,并将其克隆到PGEM-TEasy载体中进行序列分析。结果:获得高质量的胎盘组织总RNA。RT-PCR扩增出1.5kb的cDNA片段,成功构建了PGEM-T/Ang-1载体,Blast序列分析与GenBank中AY121504一致。结论:从人的胎盘中克隆Ang-1基因无突变,满足为进一步研构建pPIC9K/Ang-1毕氏酵母表达载体和表达蛋白质的需要。AIM:To clone angiopoietin-1 (Ang-1)gene from human placenta,and analyze its structural characteristics. METHODS:Total RNA was isolated from human placenta,and then the Ang-1 cDNA fragment was amplified by reverse transcription-polymerase chain reaction(RT-PCR),and it was cloned into PGEM-T Easy vector for sequence analysis. RESULTS:High quality total RNA was isolated from human placenta. A 1.5 kb fragment of cDNA was amplified by RT-PCR,and PGEM-T/Ang-1 vector was successfully established. Blast sequence analysis was coincident with AY121504 in GenBank. CONCLUSION:Ang-1 cloned from human placenta has no mutation,which satisfies the needs for further study of establishing pPIC9K/Ang-1 Pichia Pastoris expression vector and expression protein.

关 键 词：基因表达 反转录聚合酶链反应 克隆 分子 胎盘 新生血管化 病理性/病因学 血管生成因子/遗传学 遗传载体 

分 类 号：R587.1[医药卫生—内分泌]