康复期传染性非典型肺炎患者T淋巴细胞亚群水平与年龄、性别、康复时间及肺弥散功能的关系:227例随访  

Association of T-lymphocyte subpopulation level with age,gender,rehabilitative duration and lung diffusion function in patients with severe acute respiratory syndrome during rehabilitative period:a follow-up of 227 cases

作　　者：磨国鑫[1] 解立新[1] 范保星[1] 邓新立[2] 秦小玲[2] 刘又宁[1] 

机构地区：[1]解放军总医院呼吸科,北京市100853 [2]解放军总医院临床检验科,北京市100853

出　　处：《中国临床康复》2005年第7期90-92,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家高科技研究发展计划课题(2003AA208107)~~

摘　　要：目的:研究传染性非典型肺炎(severeacuterespiratorysyndrome,SARS)患者康复期的T淋巴细胞亚群变化规律,为评价SARS患者康复期细胞免疫水平提供相关参考依据。方法:本研究于2003-07/12在解放军总医院呼吸科完成。同期本院门诊随诊的北京地区临床确诊SARS康复者患者292例。符合标准的SARS患者227例,男92例,女135例。227例SARS康复者中SARS冠状病毒特异性IgG抗体阳性组(抗体阳性组)患者181例,SARS冠状病毒特异性IgG抗体阴性组(抗体阴性组)患者46例。对抗体阳性组患者检查肺功能,留取患者抗凝血,通过流式细胞仪检测CD3,CD4,CD8,CD4/CD8,对T淋巴细胞亚群水平与年龄、性别、康复时间以及肺弥散功能的关系进行统计分析。结果:抗体阳性组患者康复期CD8检测结果犤(677±182)个/μL犦明显高于抗体阴性组犤(582±247)个/μL犦(t=2.0862,P<0.05),而CD3,CD4指标与抗体阴性组比较,差异无显著性意义(P>0.05)。抗体阳性组康复时间较短患者的CD8指标高于康复时间较长的患者(t=2.2587,P<0.05),而抗体阳性组CD4/CD8指标低于抗体阴性组(t=2.0098,P<0.05)。抗体阳性组中有肺弥散功能损伤的SARS患者在康复期CD3,CD4,CD指标均明显高于弥散功能正常的SARS患者。女性和高年龄SARS康复期患者(≥37岁)的CD4指标检测结果明显高于男?AIM: To investigate the changes of T-lymphocyte subpopulations of severe acute respiratory syndrome(SARS) patients in rehabilitation period, and provide correlative reference data to evaluate the cellular immunity level of SARS patients in rehabilitation period. METHODS:The experiment was finished in the Department of Respiratory Diseases,General Hospital of Chinese PLA between July and December 2003.292 SARS rehabilitees in Beijing were followed up at the same period.Totally 227 rehabilitees(male: 92 cases, female:135 cases) who were diagnosed as having SARS,met the standard. Among 227 SARS rehabilitees, positive group of SARS coronavirus immunoglobulin G(IgG) antibody test had 181 cases and negative group of SARS coronavirus IgG antibody test had 46 cases.The pulmonary function of positive group was determined. Blood coagulatlon in patients was inhibited. The cell count of CD3,CD4,CD8 and CD4/CD8 were detected with flow cytometer. In addition, the relationship between the changes of T-lymphocyte subpopulations and the different clinical characteristics of positive group including age, sex, rehabilitation time, function of diffusion of lung were analyzed. RESULTS: The CD8 index in positive group of SARS coronavirus IgG antibody [(677±182/μL)] was significantly higher than that[(582 ±247/μL] in negative group (t=2.086 2, P< 0.05), but there was nosignificant difference(P >0.05) in the CD4 and CD3 cells between the positive group and the negative group. And In the positive group, the CD4/CD8 ratio was lower than that of the negative group. The CD8 index in short rehabilitation time of patients was higher than that of long rehabilitation time(t=2.258 7, P < 0.05).The CD4/ CD8 index in positive group was lower than that in negative group(t=2.009 8, P < 0.05).The CD3,CD4, CD8 in SARS patients with injured function of diffusion of lung during the rehabilitation period in the positive group showed a higher level than those with normal function of diffusion of lung. The CD4 index in female and older (≥37 years

关 键 词：严重急性呼吸综合征 康复 淋巴细胞亚群 抗原 CD3 抗原 CD4 抗原 CD8 CD4-CDr比值 

分 类 号：R563.1[医药卫生—呼吸系统]