家猪慢性冬眠心肌中C-JunN末端蛋白激酶1/2蛋白的表达及意义(英文)  

Expression and significance of c-Jun N-terminal protein kinase 1/2 protein in chronic hibernated myocardium of domestic pigs

作　　者：李东野[1] 朱红[1] 夏勇[1] 潘德峰[1] 杨煜[1] 李雷[1] 祁春梅[1] 

机构地区：[1]徐州医学院附属医院心内科,江苏省徐州市221002

出　　处：《中国临床康复》2005年第7期182-183,F003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省科技厅应用基础研究项目(BJ2000059)~~

摘　　要：背景:急性心肌缺血时c-JunN末端蛋白激酶(c-JunN-terminalproteinkinase,JNK)被激活,并使得缺血损伤加重。慢性冬眠心肌组织中JNK的亚型———JNK1/2是否被激活及其在慢性冬眠心肌发生发展机制中的作用又是什么呢?目的:明确慢性冬眠心肌组织中JNK1/2的蛋白表达及其磷酸化(p-JNK1/2)的变化。设计:随机对照的实验研究。单位:徐州医学院附属医院心血管病研究所。材料和方法:在徐州医学院附属医院导管室进行动物模型的制备、在徐州医学院生化教研室测定JNK1/2的蛋白表达及其磷酸化(p-JNK1/2)的变化。将14只小型中国家猪随机分为实验组(n=8)与对照组(n=6)。实验组以右冠状动脉为靶血管,经右股动脉送入自制的缩窄器,制备成慢性冬眠心肌及心肌梗死的模型。获取对照组心肌组织、实验组中的正常心肌组织及慢性冬眠心肌组织的样本进行光镜、电镜检查并采用免疫印迹(Westernblotting)分析3组心肌组织的JNK1/2的蛋白表达及其磷酸化的变化。主要观察指标:慢性冬眠心肌组织中JNK1/2是否被活化。结果:实验组慢性冬眠心肌组织p-JNK1/2比实验组正常心肌组织、对照组心肌组织高。对照组、实验组正常心肌组织、实验组慢性冬眠心肌组织p-JNK1/2的免疫活性分别为1,1.42±0.52,2.6±0.59。BACKGROUND:Acute myocardial ischemia can activate the c-Jun N-terminal protein kinase(JNK)and,in turn,the ischemia damage can be aggravated by JNK.While in chronic hibernating myocardium,whether chronic myocardial ischemia can activate JNK1/2 or not and what is the role of JNK1/2 in developing the chronic hibernating myocardium, is not clear. OBJECTIVE:To identify protein expression of JNK1/2 and the p-JNK1/2 changes in chronic hibernating myocardium. DESIGN:Randomly controlled experimental research. SETTING:This study was performed in Cardiovascular Disease Institute,the Affiliated Hospital of Xuzhou Medical College. MATERIALS:Animal models were prepared in the Catheter Department of the Affiliated Hospital of Xuzhou Medical College,protein expression of JNK1/2 and the phosphorylation(p-JNK1/2) changes were measured in the Department of Biochemistry of Xuzhou Medical College.Fourteen small Chinese domestic pigs were randomly divided into two groups,experiment group(n=8) and control group(n=6).Right coronary artery was selected as target vessel in experiment group,and home-made constrictor was delivered through right femoral artery to induce chronic hibernating myocardium and myocardial infarction. MAIN OUTCOME MEASURES:Normal myocardial tissue in control group,normal myocardial tissue and chronic hibernating myocardium sample in experiment group were checked with light microscope and electron microscope,protein expression of JNK1/2 and the phosphorylation changes in myocardium tissue of three groups were analyzed by immunoblotting (Western blotting). RESULTS:p-JNK1/2 was higher in chronic hibernating myocardium of experiment group than that of normal myocardium in experiment and control group.Immunological activity of p-JNK1/2 in control group,normal myocardium,and chronic hibernating myocardium in experiment group was 1,1.42±0.52,2.6±0.59,respectively. CONCLUSION:JNK1/2 can be activated in chronic hibernating myocardium tissue and also participated in its occurrence and development.

关 键 词：疾病模型 动物 心肌缺血/病理学 蛋白激酶类 

分 类 号：R542.2[医药卫生—心血管疾病]