针刺手厥阴心包经穴对心肌缺血再灌注损伤细胞凋亡的影响(英文)  

Effects of acupoints on pericardium meridian of hand-Jueyin with acupuncture on cell apoptosis induced by myocardial ischemic reperfused injury

作　　者：田岳凤[1] 吴富东[2] 乔海法[2] 韩秀珍[2] 单秋华[2] 

机构地区：[1]山西中医学院针灸推拿系,山西省太原市030024 [2]山东中医药大学针灸推拿学院,山东省济南市210045

出　　处：《中国临床康复》2005年第7期195-197,共3页Chinese Journal of Clinical Rehabilitation

基　　金：山东省科技厅资助项目(021100101)~~

摘　　要：背景:心肌细胞在缺血再灌注损伤的过程中会加速细胞的凋亡,针刺手厥阴经穴位可抑制再灌注损伤的进程,减少凋亡的心肌细胞,从而呈现出对心脏的良好保护作用。目的:观察针刺手厥阴经穴对心肌缺血再灌注损伤过程中心肌细胞凋亡的影响。设计:以实验动物为研究对象的随机对照实验。单位:一所中医高等院校的针灸推拿系。材料:实验于2001-04/12在山东中医药大学中心实验室完成。选用Wistar大鼠40只,随机分为5组:空白组、模型组、针刺“内关”治疗组、针刺“郄门”治疗组、针刺“支沟”对照组。方法:电针大鼠心包经穴位20min后,结扎左冠状动脉前降支40min,心电图监测,再电针穴位20min,松扎,恢复灌流60min,摘取心脏,制成细胞悬液,流式细胞仪检测。主要观测指标:针刺对心肌细胞凋亡率变化的影响。结果:模型组出现典型的凋亡峰,凋亡率明显升高,针刺心包经穴组与模型组比较差异均有非常显著意义(P<0.01),其中针刺“内关”治疗组、针刺“郄门”治疗组、针刺“支沟”对照组及模型组的凋亡率分别为(4.29±0.76)%,(5.17±0.88)%,(13.96±2.77)%,(16.01±1.04)%。结论:针刺手厥阴经穴能有效地抑制心肌细胞的凋亡,从而减轻由于凋亡细胞导致的心肌损伤。BACKGROUND:Apoptosis of cardiomyocytes is speeded up during ischemic reperfused injury.Acupuncture on the points of hand-Jueyin meridian can inhibit the progression of reperfused injury and reduce apoptosis of cardiomyocytes so as to provide the protection on the heart. OBJECTIVE: To observe the effects of acupuncture on the points of hand-Jueyin meridian on apoptosis of cardiomyocytes during ischemic reperfused injury. DESIGN:Randomized controlled experiment based on the experimental animals. SETTING:Department of acupuncture and massage in a university of traditional Chinese medicine. MATERIALS:The experiment was performed in Central Laboratory of Shandong University of Traditional Chinese Medicine from April to December 2001.Forty Wistar male rats were employed,and were randomized into 5 groups,named as blank group, model group,“Neiguan(PC 6)”group,“Ximen(PC 4)”group and “Zhigou(TE 6)”group. METHODS:After electric acupuncture on the points of pericardium meridian for 20 minutes,the ligature was done on the anterior-descending branch of the left coronary artery for 40 minutes.Electrocardiogram (ECG) monitoring was followed.The ligature was released after electric acupuncture on the points for another 20 minutes and perfusion was restored for 60 minutes.Afterward,the heart was extracted,the cellular suspension was prepared and the determination with flow-type cell apparatus was applied. MAIN OUTCOME MEASURES:Effects of acupuncture on the changes in apoptosis rate of cardiomyocytes. RESULTS:In the model group,the typical apoptosis peak presented, the apoptosis rate was remarkably increased.The comparison between the points of pericardium meridian groups and the model group indicated very significant difference(P< 0.01).The apoptosis rates in “Neiguan(PC 6)”group,“Ximen(PC 4)”group and “Zhigou(TE 6)”group as well as the model group were(4.29±0.76)%, (5.17±0.88)%,(13.96±2.77)%and (16.01±1.04)%respectively. CONCLUSION:Acupuncture on the points of hand-Jueyin meridian inhibits myoc

关 键 词：心肌再灌注损伤 细胞死亡 针刺 穴位 心包经 

分 类 号：R542.2[医药卫生—心血管疾病]