氟伐他汀对大鼠肺间质纤维化及通气功能的影响(英文)  

Effect of fluvastatin on pulmonary interstitial fibrosis and ventilation function in rats

作　　者：赵峰[1] 戚好文[1] 赵一玲[2] 吕荣[3] 宋立强[1] 叶江枫[1] 

机构地区：[1]解放军第四军医大学西京医院呼吸内科,陕西省西安市710032 [2]解放军第四军医大学基础部病理学教研室,陕西省西安市710032 [3]解放军第四军医大学西京医院骨科研究所,陕西省西安市710032

出　　处：《中国临床康复》2005年第7期201-203,F003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:肺间质纤维化病理上可见以成纤维细胞(fibroblast,Fb)为主的大量间质细胞的增生及胶原分泌的增多,氟伐他汀对Fb等多种细胞具有增殖抑制作用。目的:观察氟伐他汀对体外培养的大鼠肺Fb增殖的抑制作用和对博来霉素诱发的大鼠肺纤维化及肺通气功能的影响。单位:解放军第四军医大学西京医院呼吸内科;解放军第四军医大学基础部病理学教研室;解放军第四军医大学西京医院骨科研究所。设计:随机对照实验研究。材料:本实验于200101/12在解放军第四军医大学西京医院呼吸内科实验室完成。实验选用健康雄性一级SD大鼠31只。干预:取1只大鼠,摘取其肺组织,体外培养大鼠肺Fb,加入氟伐他汀,MTT法观察对培养细胞生长曲线的影响及不同浓度(0,1×10-7,1×10-6,1×10-5,1×10-4,1×10-3,1×10-2mol/L,氟伐他汀0mol/L为空白对照组)的氟伐他汀对培养细胞的抑制作用;计数法观察氟伐他汀对大鼠肺Fb分裂指数的影响;羟脯氨酸比色法检测氟伐他汀对培养的大鼠肺Fb胶原分泌的影响。随机将另30只大鼠分为正常对照组、博来霉素组及氟伐他汀组。氟伐他汀组根据治疗开始时间为模型制备后第1,15天和给药剂量为20,10mg/kg(高、低剂量)分为4个治疗组:第1天高、低剂量组,第15天高、低剂量组。28d后处死动物,肺组织作HE、Masson和苦味酸天?BACKGROUND:The pathological characteristics of pulmonary interstitial fibrosis are the proliferation of a large number of fibroblasts and the increasing deposition of matrix collagen that takes the place of normal lung structure.Fluvastatin can inhibit the proliferation of fibroblasts and many other cells. OBJECTIVE:To investigate the effects of fluvastatin in inhibiting the proliferation of rat lung fibroblasts cultured in vitro and its influence on bleomycin-induced pulmonary fibrosis and ventilation function. DESIGN:A randomized controlled trial. SETTING:Department of Respiratory Diseases,Xijing Hospital, Fourth Military Medical University of Chinese PLA;Teaching and Research Section of Pathology,Department of Basic Medicine, Fourth Military Medical University of Chinese PLA;Research Institute of Orthopedics,Xijing Hospital,Fourth Military Medical University of Chinese PLA. PARTICIPANTS:The study was conducted in the laboratory of Department of Respiratory Diseases,Xijing Hospital of Fourth Military Medical University of Chinese PLA from January to December 2001. Thirty-one healthy adult male Sprague-Dawley(SD) rats of grade I were selected in this study. INTERVENTIONS:The fibroblasts derived from the lung normal of one rat were cultured in vitro in media containing fluvastatin.The effect of fluvastatin on the growth curve and the effect of its different concentrations(0, 1×10-7,1×10-6,1×10-5,1×10-4,1×10-3 and 1×10-2 mol/L,fluvastatin of 0 mol/L was taken as the blank control group) in inhibiting the cultured cells were observed with MTT colorimetry.The effect of fluvastatin on the division index of the fibroblasts was analyzed by direct cell counting.Hydroxyproline colorimetry was used to detect the influence of fluvastatin on the collagen secretion in the media.The other 30 SD rats were divided into six groups:normal control group, bleomycin-induced group and fluvastatin-treated groups(TH1,TL1, TH15 and TL15 groups) named according to the date of giving fluvastatin,i.e.the 1st day and the 15th day

关 键 词：氟伐他汀 肺疾病/间质性 成纤维细胞 

分 类 号：R563[医药卫生—呼吸系统]