3种不同致伤条件下在体肺泡巨噬细胞的CD68和TNF-α表达  

Expressions of CD68 and TNF-α on alveolar macrophages in post-injured rats

作　　者：温建良[1] 谢志坚[1] 吕凤林[1] 李磊[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤感染研究室,重庆400042

出　　处：《第三军医大学学报》2005年第3期217-219,共3页Journal of Third Military Medical University

摘　　要：目的　探讨不同致伤条件对继发性肺损伤肺泡巨噬细胞CD68和TNF α表达的影响。方法　利用免疫组化方法 ,将两种抗体进行双标染色 ,观察大鼠在创伤、LPS腹腔感染、创伤复合LPS腹腔感染时与正常情况下肺泡巨噬细胞阳性表达情况 ,结合各组死亡率和常规HE染色进行分析。结果　肺泡巨噬细胞CD68和TNF α阳性表达细胞在伤后均明显增加 ,与对照组差异显著 (P <0 0 1) ,各致伤组间也有差异 (P <0 0 1) ,创伤复合LPS腹腔感染组增加最显著 ,LPS腹腔感染组次之 ,创伤组最少。结论　肺泡巨噬细胞CD68和TNF α的表达受致伤因素的影响 ,其表达强度随伤情加重而增强。Objective To study the expressions of CD68 and TNF-α on alveolar macrophages (AMs) in rats injured by different wound factors. Methods CD68 and TNF-α were detected by double-labelling immunohistochemical method in four groups (normal, trauma, LPS, trauma and LPS). Results CD68 and TNF-α positive cells increased significantly in three injured groups (P<0.01). There was significant difference in CD68 and TNF-α positive cells as compared with the control group (P<0.01). The most significant increase was observed in the groups of trauma and LPS, followed by LPS group. The lowest level of CD68 and TNF-α positive cells was found in the trauma group. Conclusion The expression of CD68 and TNF-α on AMs can be affected by different wound factors. The intensity of expression may be enhanced in a severity-dependent manner.

关 键 词：肺泡巨噬细胞 CD68 TNF-Α 

分 类 号：R322.35[医药卫生—人体解剖和组织胚胎学] R392.12[医药卫生—基础医学]