RDP1258对人外周血单核细胞增殖能力及HO-1活性的影响  

Effects of RDP1258 on proliferation and heme oxygenase-1 activities of human peripheral blood mononuclear cells

作　　者：易善红[1] 宋波[2] 汪泽厚[3] 

机构地区：[1]第三军医大学新桥医院泌尿外科,全军肾脏病中心,重庆400037 [2]第三军医大学西南医院全军泌尿外科中心,重庆400038 [3]第三军医大学解放军空军总医院泌尿外科,北京100036

出　　处：《第三军医大学学报》2005年第3期230-233,共4页Journal of Third Military Medical University

基　　金：国家新药基金资助项目 ( 96 90 10 5184 )~~

摘　　要：目的　观察一种新型HLA衍生肽 (RDP12 5 8)针对人外周血单核细胞由丝裂原以及同种异体抗原刺激导致的增殖能力的影响 ,并探讨其机制。方法　人工固相合成RDP12 5 8,3 H TdR掺入法观察其在体外对人外周单核细胞增殖反应的影响 ,以酶化学法观察其对淋巴细胞血红素氧合酶 1(hemeoxygenase 1,HO 1)活性的影响。结果　RDP12 5 8可显著抑制人外周血单核细胞的转化及MLR增殖反应 ;该肽体外降低HO 1活性呈现出剂量依赖性。结论　RDP12 5 8能够显著抑制人外周单核细胞经丝裂原或同种抗原刺激引起的增殖反应 ,这种作用可能是通过影响HO 1活性而达到的。Objective To observe the effects of a novel HLA-derived peptide, RDP1258, on the human peripheral blood mononuclear cell (PBMC) proliferation to ConA and MLR, and to investigate the mechanisms. Methods Peptide RDP1258 was chemically synthesized. The effects of the peptide on alloreactive cytotoxic activities of human PBMCs were observed using 3HTdR incorporation method. RDP1258, HLA-B2702.75-84, and control peptide were administrated respectively in every experiment. The activity of heme oxygenase-1 (HO-1) was analyzed by the enzymochemical method. Results The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of human PBMCs and inhibited HO activity in a dose-dependent manner in vitro. Conclusion HO-1 might participate in the inhibitory effect of RDP1258 on the proliferation of human PBMCs induced by mitogen and isoantigen.

关 键 词：HLA衍生肽 混合淋巴细胞反应 血红素氧合酶-1 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R331.14[医药卫生—基础医学]