长期低剂量米非司酮对大鼠卵巢超微结构和内分泌功能的影响  

Effects of long-term administration of lower-dosage mifepristone on ultrastructure and incretion of rat ovary

作　　者：朱顺法[1] 方马荣[2] 金理正[1] 

机构地区：[1]台州学院医学院,台州318000 [2]浙江大学医学院

出　　处：《解剖学杂志》2005年第1期14-17,共4页Chinese Journal of Anatomy

基　　金：20 0 3年台州市科技计划项目基金

摘　　要：目的 :观察不同时间、不同小剂量米非司酮 (RU4 86 )对大鼠卵巢超微结构形态和功能的影响 ,研究其避孕机制。方法 :实验组大鼠分别给不同剂量药 35、92d ,动态测定血清中雌孕激素水平 ,透射电镜观察卵巢 ,扫描电镜观察子宫内膜上皮。结果 :各实验组血清雌二醇水平较对照组下降 ,血清孕酮水平较对照组升高 ,随时间延长加剧 ,呈剂量依赖性。多数卵泡闭锁 ,部分形成间质腺 ,基质中成纤维细胞、胶原纤维增多 ,其超微病理改变随用药时间延长加重 ;大鼠子宫内膜纤毛上皮明显减少 ,腺上皮分泌抑制。结论 :小剂量RU4 86对卵泡发育有抑制作用 ,能影响卵巢分泌类固醇激素 ,并对卵巢皮质细胞有一定程度损伤 ,长期使用可能对子宫上皮直接抑制。Objective: To elucidate the mechanism of contraception by mifepristone. Methods:The effects of long periods treatment with the mefepristone on ovarian ultrastructures and function of mature rats were investigated with electron microscopy and by an assay of ovarian steroids.Mifepristone was administrated intragastrically in 3 groups for 35 d and 92 d respectively.The dynamical levels of serum steroids were monitored;the ultrastructure of follicles, interstitial glands and cells of matrix in ovariocortex were observed with TEM;the uterine epithelial cells were scanned with SEM.Results:Long-time in vivo administration in the mature rats results in decrease in serum estradiol levels, increase in serum progesterone levels and follicular atresia, decrease in the total number of follicles and partial atresia follicles formed into interstitial glands.Fibroblast and collagen increasesed in ovarian matrix. The uterine luminal epithelium lost its cilia after 35 d or 92 d treatment with progesterone antagonist. Exacerbation of pathological alteration gradually developed with prolongation of administrational period. Conclusion:Long-term administration of lower-dosage mifepristone inhibits ovarian folliculogenesis and is injurious to the cells of ovariocortex to some degree. It also suppresses estradiol secretion and stimulates the progesterone production.The direct inhibitory action of the progesterone antagonist on uterine epithelial cells should be taken into account when treating women in their reproductive years with these drugs for long periods.

关 键 词：大鼠 上皮 卵巢 米非司酮 对照组 超微结构 低剂量 基质 用药时间 卵泡发育 

分 类 号：R965[医药卫生—药理学]