等吸收双波长分光光度法同时测定芦荟中芦荟大黄素和芦荟甙  

Determination of Aloe-Emodin and Aloin without Separation by Dual-Wavelength Spectrophotometry

作　　者：黄丽英[1] 林新华[1] 赖丽旻[1] 郑艳洁[1] 刘思艺[1] 

机构地区：[1]福建医科大学药学院,福州350004

出　　处：《福建医科大学学报》2005年第1期58-60,64,共4页Journal of Fujian Medical University

基　　金：福建省科技厅重点资助项目 (2 0 0 2 I0 15 )

摘　　要：目的　建立紫外可见分光光度法同时测定芦荟大黄素和芦荟甙的方法。　方法　根据双波长原理 ,分别在测定波长 2 5 5 ,376 nm ,参比波长 35 5 ,4 75 nm处测定芦荟大黄素和芦荟甙含量。　结果　芦荟大黄素在 2 .0 0× 10 - 6 ～ 5 .0 0× 10 - 5mol/ L浓度范围内与光密度 (D)呈线性关系 ,r=0 .9995 ;芦荟甙在 2 .0 0× 10 - 6 ～ 6 .0 0× 10 - 5mol/ L浓度范围内与 D呈线性关系 ,r=0 .9998。　结论　等吸收双波长分光光度法操作快速、简便、准确 ,无需分离可同时测定芦荟样品中两组分含量。Objective To develope a method for determination of Aloe-Emodin and also Aloin in Aloe without separation dual-wavelengths UV spectrophotometry. Methods Wavelengths were selected at 255 nm and 355 nm, and the reference wavelengths at 376 nm and 475 nm for Aloe-Emodin and Aloin respectively.Results The linearity correction of content and optics density in Aloe-Emodin was 2.00×10 -6～5.00×10 -5mol/L (r=0.9995) and that in Aloin was 2.00×10 -6～6.00×10 -5mol/L (r=0.9998). Conclusion This method is simple, convenient, accurate and could be used for the determination of Aloe-Emodin and also Aloin in Aloe sample.

关 键 词：芦荟大黄素 芦荟甙 芦荟 分光光度法 

分 类 号：R284.1[医药卫生—中药学]