血管平滑肌细胞在几种基质膜片上生长情况及三种接种培养方法的比较  

The comparison of vascular smooth muscles cells in degradable materials and different seeding methods of the cells

作　　者：熊猛[1] 艾玉峰[1] 王甦 鲁开化[1] 袁文周[1] 夏文森[1] 

机构地区：[1]第四军医大学西京医院整形外科中心,陕西西安710032 [2]沈阳军区总医院整形科

出　　处：《中国实用美容整形外科杂志》2005年第1期54-57,共4页

基　　金：国家自然科学基金资助项目 (3 0 0 70 777)

摘　　要：目的　筛选适合于组织工程血管构建的可降解基质材料及最佳的接种培养方式。方法　将血管平滑肌细胞接种于几种现有的可降解基质材料上 ,采用MTT法观察细胞在几种基质材料上的生长情况 ;比较静态、水平摇床、旋转接种三种接种培养方式。结果　细胞相容性比较好的材料有 :PGA、胶原加黏多糖、脱钙骨等。三种接种方法的接种率为 :静态 1.5 % ,水平摇床 7.2 % ,旋转接种培养 5 3.5 %。结论　PGA、胶原加黏多糖、脱钙骨三种材料适合于组织工程血管再造的应用。而三种接种培养方式以旋转接种培养的效率最高。Objective To select degradable materials and best seeding method for tissue engineering blood vessel. Methods Vascular smooth muscles cells were seeded onto these degradable scaffolds, MTT method was used to analyze the growth state of the co-cultured cells. Optimizing seeding methods were also be compared. Results SMCs grew better on PGA, collage combined with GAG and decalcifying bone, than the others. The efficiency of seeding is: static culture 1.5%; stired culture 7.2%; dynamic culture 53.5%. Conclusion PGA, collage combined with GAG, decalcifying bone are relatively compatible with the cells; Dynamic rotation seeding culture has highest seeding efficiency.

关 键 词：血管平滑肌细胞 可降解材料 组织工程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]