间充质干细胞对T淋巴细胞转化的影响  

The influence of mesenchymal stem cell on T lymphocyte transformation

作　　者：江小霞[1] 张毅[1] 李秀森[1] 吴英[1] 于晓妉[1] 唐佩弦[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《解放军医学杂志》2005年第2期130-132,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金 (编号 30 2 71 2 4 5;30 4 70 640 );国家 973资助(编号G1 9990 5430 2 );国家 863 (编号 2 0 0 3AA2 0 51 70 )资助课题

摘　　要：目的　研究间充质干细胞(mesenchymalstemcell,MSC)对T细胞的免疫调节作用。方法　从骨髓中分离培养MSC ,从外周血中分离单个核细胞 ,进一步用免疫磁珠纯化CD3+ T细胞 ,观察MSC对T淋巴细胞转化的影响 ;且用ELISA实验检测上清中IFN γ和IL 10的表达。结果　当CD3+ T细胞量为 2× 10 5/孔时 ,MSC对其增殖反应主要表现为负调控 ,且随MSC细胞量的增多 ,这种负调控越明显 ;而当体系中CD3+ T细胞量为 5× 10 4 /孔时 ,MSC对其增殖反应表现为正调控 ,而且随MSC细胞量的增多这种促进作用越明显。ELISA结果表明 ,在没有MSC的存在下 ,2× 10 5/孔的T细胞分泌细胞因子IFN γ和IL 10均高于 5× 10 4/孔的T细胞 ;但在有MSC存在情况下 ,2× 10 5/孔的T细胞分泌细胞因子IFN γ和IL 10反而低于 5× 10 4/孔的T细胞。结论　MSC对T细胞的调控是复杂的 ,不仅与MSC的细胞量有关 ,还与T细胞的量有关。Objective To study the immunoregulatory ability of mesenchymal stem cell (MSC) on T cells. Methods MSC were isolated and cultured from bone marrow and CD3 + T cells from peripheral blood. Then the action of MSC on T cell proliferation was investigated. ELISA assay tested IFN-γ and IL-10 secretion of CD3 + T cells. Results The data showed that MSC inhibited T cell proliferation in a dose dependent manner when there was 2×10 5/well (96-well plate) CD3 +T cells. While MSC stimulated T cell proliferation when there was 5×10 4/well CD3 + T cells. High dose CD3 +T cells produced high level IFN-γ and IL-10 in the absence of MSC, while when there were MSC in the culture, 5×10 4/well CD3 +T cells secreted more IFN-γ and IL-10 than 2×10 5/well CD3 + T cells. Conclusion The result suggested that the effect of MSC on T cells was complex, not only correlating with the cell concentration of MSC but also of T cells.

关 键 词：CD3^+T细胞 IL-10 T淋巴细胞 MSC 分泌细胞因子 增殖反应 调控 干细胞 免疫磁珠 转化 

分 类 号：R392[医药卫生—免疫学] R562.25[医药卫生—基础医学]