G-CSF受体及其mRNA在结直肠癌和其附近正常黏膜中的表达  

Expression of G-CSFR and its mRNA in human colorectal cancer and normal mucosa tissues

作　　者：杨小冬[1] 刘福坤[1] 许哲[1] 陈彻[1] 吴晓宇[1] 李刚[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院普通外科研究所,南京210002

出　　处：《解放军医学杂志》2005年第2期149-152,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的　研究粒细胞集落刺激因子受体 (G CSFR)在结直肠癌中的表达情况 ,了解G CSF在结直肠癌发生、发展中的作用。方法　应用免疫组化RT PCR法分别检测 4 2例结直肠癌及其附近正常黏膜组织中G CSFR蛋白和其mRNA表达情况 ,并分析结直肠癌肿瘤细胞G CSFR的表达及与临床病理因素的关系。结果　免疫组化表明结直肠癌中有明显的G CSFR蛋白的表达 (31/ 4 2 ,73 81% ) ,并明显高于正常黏膜 (2 0 / 4 2 ,4 7 6 2 % ,P <0 0 0 1) ,许多G CSFR表达强阳性的结直肠癌 ,相应的正常黏膜G CSFR阴性或弱阳性表达。G CSFR的表达与肿瘤病理分期、分化程度相关(P =0 0 0 1、P <0 0 0 1) ,与病人性别、年龄、肿瘤大小无明显相关 (P =0 346、P =0 6 86、P=0 4 5 9)。RT PCR结果示 4 2例肿瘤标本中 2 1例呈G CSFRmRNA阳性表达 ,而相应正常黏膜仅 11例。结论　G CSFR在结直肠癌中的表达较正常黏膜明显上调 ,结直肠癌患者应用G CSF时应考虑其对肿瘤细胞的影响。Objective The purpose of the study was to study the expression of granulocyte colony-stimulating factor receptor (G-CSFR) in human colorectal cancer (CPC) and the role of granulocyte colony-stimulating factor (G-CSF) in the development and progression of human CRC. Methods Forty-two specimens of CRC and normal colorectal mucosa were harvested from colon or rectum in a group of patients who were suffering from CRC. Immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) technique were used to demonstrate expression of granulocyte colony-stimulating factor receptor. The relationship between expression of G-CSFR and clinical or pathological pictures was analyzed. Results Immucohistochemical analyses revealed that G-CSFR was expressed in the human colorectal cancer (25/42, 59.52%), and it appeared to be up-regulated compared with the normal mucosa (14/42, 33.33%, P<0.001). In sharp contrast with strong expression of G-CSFR in many CRC, the normal colorectal mucosa of these patients showed negative or only weakly positive expression. A significant correlation was found between G-CSFR expression and tumor stage (P=0.001), tumor differentiation (P<0.001), and there are no statistically significant relationship between the expression of G-CSFR and the age, gender and tumor size (P=0.346, P=0.686, P=0.459). The results of RT-PCR showed that twenty-one of all forty-two tumors were positive for G-CSFR mRNA expression, while only eleven out of forty-two normal colorectal mucosa were so. Conclusions Our data presented here indicates that granulocyte colony-stimulating factor receptor is frequently expressed in human colorectal cancers, thus supporting a hypothesis that granulocyte colony-stimulating factor plays a role in the physiology of CRC.

关 键 词：受体 粒细胞集落刺激因子 结肠直肠肿瘤 免疫组织化学 逆转录聚合酶链反应 

分 类 号：R392.114[医药卫生—免疫学] R735.35[医药卫生—基础医学]