蝎毒对小鼠淋巴瘤细胞株的生长抑制和诱导凋亡作用的研究  

Studies of BMK on inhibition of cell growth and induction of apoptosis in Yac-1 cell line

作　　者：贾莉[1] 孟秀香[1] 孙宏丹[1] 宋振岚[1] 孔力[2] 赵瑾瑶[2] 

机构地区：[1]大连医科大学临床血液教研室,辽宁大连116027 [2]大连医科大学组胚教研室,辽宁大连116027

出　　处：《大连医科大学学报》2001年第3期164-166,共3页Journal of Dalian Medical University

摘　　要：目的 :研究蝎毒对小鼠淋巴瘤细胞株的生长抑制和诱导凋亡作用。方法 :应用MTT法观察蝎毒对细胞的抑制作用 ,光镜、透射电镜观察细胞结构的改变 ,原位末端标记检测DNA断裂 ,流式细胞术分析凋亡细胞。结果 :蝎毒 (12 5 - 2 0 0 μg/ml)可明显地抑制小鼠淋巴瘤细胞生长和诱导细胞凋亡 ,并且其作用强度在一定范围内呈现对浓度和时间的依赖性。结论 :蝎毒能抑制小鼠淋巴瘤细胞生长和诱导细胞凋亡。Objective: The study of the BMK on inhibition of cell growth and induction of apoptosis in Yac-1 cell line. Methods: The growth inhibition was analysed by MTT assay, cell apoptosis by light microscopy, transmission electron microscopy, flow cytometry and TUNEL technique. Results: The results showed that BMK(12 5-200 μg/ml) could inhibit cell growth and induce apoptosis obviously, and the effect of BMK was dose- and time-dependent within a limited range. Conclusions: BMK could inhibit cell growth and induce apoptosis obviously.

关 键 词：蝎毒 细胞凋亡 小鼠淋巴瘤细胞株 

分 类 号：R285.5[医药卫生—中药学]