老年大鼠海马CA_1区锥体细胞凋亡的形态学观察  

Pathological Observation of Pyramidal Cells in CA_1 Region of the Hippocampus After Reperfusion Following Forebrain Ischemia in Old Rats

作　　者：刘学忠[1] 崔旭[2] 任建新[1] 卞建春 刘宗平[1] 王捍东[1] 王宗元[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]解放军总医院老年医学研究所,北京100853

出　　处：《扬州大学学报（农业与生命科学版）》2004年第4期72-74,共3页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展规划项目(G2000057000);扬州大学畜牧兽医学院科研基金项目(2003006)

摘　　要：采用股动脉放血并双侧颈总动脉夹闭法制作全脑缺血再灌注模型,于术后3d处死动物取脑,以常规电镜程序制作标本,观察海马CA1区锥体细胞超微结构的改变;同时结合原位末端标记法检测,探讨全脑缺血再灌注后海马CA1区锥体细胞的病理损伤形式。结果表明:与假手术相比,缺血再灌注3d后海马CA1区出现了较多的凋亡样改变的锥体细胞,证明在脑缺血再灌注损伤中,细胞凋亡是神经元死亡的一种重要形式。In order to observe the pathologic injures of pyramidal cells of CA_1 region in the hippocampus 3 d after reperfusion following 10 min forebrain ischemia, bilateral carotid arteries occlusion model of old rats were used and the changes of ultrastructure of pyramidal cells of CA_1 region in the hippocampus were observed with electron microscope; meanwhile, we observed the changes of pyramidal cells in CA_1 region of the hippocampus through Tunel (Terminal-deoxynucleotidyl transferase-mediated dUTP-biotin nick and labeling) staining. The results showed: Compared with the sham-group,the ultrastructure of pyramidal cells of CA_1 region in the hippocampus 3 d after reperfusion following 10 min forebrain ischemia showed apoptosis-like changes.it has been tested that Apoptosis may be an important form in the course of cerebral ischemia-reperfusion(IR) injury.

关 键 词：大鼠 脑缺血 海马 凋亡 形态学 

分 类 号：S852.35[农业科学—基础兽医学] R743[农业科学—兽医学]