用花药愈伤组织作为转化受体的水稻转基因植株的分析  

Analysis of the Transgenic Rice Plants Derived from Transformed Anther Calli

作　　者：蒋苏[1] 陈彩艳[2] 程祝宽[2] 蔡润[1] 翟文学[2] 朱立煌[2] 

机构地区：[1]上海交通大学农业与生物学院植物科学系,上海201101 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《Acta Genetica Sinica》2004年第12期1381-1387,共7页

摘　　要：以花药愈伤组织作为转化的受体材料 ,利用农杆菌介导法将已克隆的Xa2 1基因导入粳稻栽培品种台北30 9中。共获得 7个转基因株系 ,其中 2个单倍体 ,4个二倍体 ,1个混倍体。PCR、Southern、FISH以及白叶枯病抗性的分析结果都表明Xa2 1基因已整合到T0 代受体基因组。调查了 4个二倍体株系T1代的分离情况 ,经 χ2 测验证明 ,有 2个株系的分离比为 3∶1,为单拷贝插入 ,另外 2个株系不符合孟德尔分离。 4个T0Rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene,Xa21,into a japonica rice variety,Taipei 309,via Agrobacterium-mediated transformation.Seven green transgenic plants,including one mixoploid,two haploid,and four diploid plants,were regenerated.PCR,Southern blot,FISH and blight resistance analysis all indicated that Xa21 gene has been integrated into the T_0 plant genomes.T_1 generations of the four diploid T_0 plants were further investigated for resistance segregation.χ~2 test showed that two T_1 populations segregated with a ratio of 3∶1,indicating that a single copy of Xa21 gene was integrated into the genome,whereas the segregation ratios of the other two T_1 populations were non-Mendelian.Therefore,the four diploid transgenic plants should be heterozygous diploids.

关 键 词：水稻 花药愈伤组织 单倍体 转基因 

分 类 号：S511[农业科学—作物学]