小麦耐盐突变体盐胁迫下的蛋白质组分析  

Proteomic Analysis of the Salt Tolerance Mutant of Wheat Under Salt Stress

作　　者：霍晨敏[1] 赵宝存[1] 葛荣朝[1] 沈银柱[1] 黄占景[1] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016

出　　处：《Acta Genetica Sinica》2004年第12期1408-1414,共7页

基　　金：国家自然科学基金 (编号 :3 0 0 70 471);河北省自然科学基金 (编号 :3 0 110 3 )~~

摘　　要：首次采用双向电泳的方法分析 1%NaCl胁迫 72h的一对小麦耐盐 (RH870 6 4 9)及敏盐突变体 (H870 6 34)的蛋白质组。经过MALDI TOF分析和数据库检索发现两者在H+ ATP酶 β亚基、谷氨酰胺合成酶前体、OEC33和RuBP羧化酶小亚基等 5个候选蛋白存在质或量的差异。这 5种蛋白均为叶绿体蛋白 。Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours.After being analysised by MALDI-TOF-MS and Mascot software,the qualitative and quantitative differences were identified between the two materials for five candidate proteins:H^+-transporting two-sector (ATPase),glutamine synthetase 2 precursor, putative 33 kD oxygen evolvingprotein of photosystem II and ribulose-1,5-bisphos-phate carboxylase/oxygenase small subunit.These five proteins are all belong to chloroplast proteins.They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.

关 键 词：小麦 突变体 双向电泳 肽质指纹分析 

分 类 号：S512.1[农业科学—作物学]