重组人胰高血糖素样肽1(7-36)促进INS-1细胞胰岛素释放与合成  

Recombinant human glucagon-like peptide-1 (7-36) enhances insulin release and insulin mRNA expression in INS-1 cells

作　　者：巩秋红[1] 娄晋宁 叶丽亚 李光伟[1] 

机构地区：[1]北京中日友好医院内分泌科,100029 [2]中日友好临床研究所病理生理室

出　　处：《中华内分泌代谢杂志》2004年第6期559-560,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：国家 8 63课题基金资助项目 (2 0 0 2AA2 0 50 61 )

摘　　要：在INS 1细胞中分别加入不同浓度的葡萄糖和重组人胰高血糖素样肽 1〔rhGLP 1(7 3 6〕溶液 ,孵育 4h ,用放免法测定上清液中胰岛素含量 ,并利用RT PCR技术对INS 1细胞的胰岛素mRNA水平作半定量分析。结果提示rhGLP 1(7 3 6)不仅能促进INS细胞的胰岛素释放 ,而且能促进胰岛素基因表达。In INS-1 cells, the insulin secretion was investigated by radioimmunoassay (RIA) after 4 h incubation in medium containing different concentrations of glucose and recombinant human glucagon-like peptide-1 (rhGLP-1) (7-36). Insulin mRNA level in INS-1 cells was assessed by a semi-quantitative RT-PCR method. rhGLP-1 (7-36) is not only a powerful insulin secretagogue, but also can increase insulin gene expression in INS-1 cells.

关 键 词：胰岛素基因 INS-1细胞 胰高血糖素 GLP-1 放免法测定 促进 RT-PCR技术 mRNA水平 释放 表达 

分 类 号：R96[医药卫生—药理学]