不同固定剂对免疫组织化学染色效果的影响  

The Influencg of Different Fixatives on the Effects of Immunohisto Chemical Staining

作　　者：张大红[1] 王琳[1] 李庆明[1] 

机构地区：[1]徐州医学院,江苏徐州221002

出　　处：《实用医技杂志》2005年第01B期155-156,共2页Journal of Practical Medical Techniques

摘　　要：目的:比较不同固定剂对免疫组织化学染色的影响。方法:取大鼠胃窦组织,分别采用4 %多聚甲醛、10%甲醛和Bouin液固定,用免疫组织化学方法结合图像分析技术检测大鼠胃窦黏膜内生长抑素(SS)和胃泌素(Gas)的表达。结果:用Bouin液固定的组织经免疫组织化学染色显示的阳性细胞数比用其他2种固定剂所显示的阳性细胞数多(P<0 .0 5 ) ,而3种固定剂显示的SS和Gas的阳性细胞平均灰度值差别无显著性(P>0 .0 5 )。结论:Bouin液比4%多聚甲醛、10 %甲醛能较好保存大鼠胃窦黏膜内SS和Gas的抗原性。Objective To compare the influence of different fixatives on immunostains.Methods Samples of rat gastric antrum were respectively fixed in 4 % para for maldehyde?10 % formalin and Bouins solution to detect expression of somatostatin and gastrin in the rat antral gastric mucosa by using immunohistochemical method combined with image analysis technique.Results The number of immuno-reactive positive cells in gastric mucosa fixed in Bouins solution were more than those fixed in 4 % paraformaldehyde and 10 % formalin(P<0.05). There were no marked differences among the positive cell grey levels using three fixatives(P>0.05).Conclusions Bouins solution could preserve more antigen property of somatostatin and gastrin than 4 % paraformaldehyde and 10 % formalin.

关 键 词：固定剂 免疫组织化学 胃窦黏膜 大鼠 

分 类 号：R446.6[医药卫生—诊断学]