不同冻存方法对脐血造血细胞的影响  

The Effects of Using Different Methods on Preservation of CordBlood Hematopoietic Cells

作　　者：白艳艳[1] 缪竞诚[1] 张占英[1] 张澜生[1] 

机构地区：[1]苏州大学基础医学系 细胞免疫中心,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2004年第6期839-840,共2页Suzhou University Journal of Medical Science

摘　　要：目的 探讨不同冻存方法对脐血造血细胞的影响。方法 冻存前后脐血单个核细胞通过台盼蓝拒染率、对K562杀伤活性及总集落形成数来进行检测。结果 控制冻存保护剂浓度和程序性降温可减小对脐血造血细胞的损害。结论 该研究可为脐血建库提供一种较好的冻存方法。Objective To show the effects of different methods on preservation of cord blood hematopoietic cells below zero degree. Methods MNCs were isolated with Ficoll, and the dectection included methods of observing Trpanblue dying, killing activity to K562 cells, and CFU total number. Results We were able to benefit from controlling the v/v of DMSO and gradually lowering the temperature of frozen cells. Conclusion The results can help us to select a good method when a cord blood bank is established.

关 键 词：脐血 造血细胞 冻存 

分 类 号：R392-33[医药卫生—免疫学]