猪外周血内皮祖细胞的分离培养和鉴定  

Isolation and Culture of Endothelial Progenitor Cells from Pig Peripheral Blood

作　　者：陈书艳[1] 王飞[1] 周卿[1] 颜雪芸[1] 许勤[1] 舒红[1] 荣烨之[1] 

机构地区：[1]上海第二医科大学附属新华医院心内科,上海200092

出　　处：《细胞生物学杂志》2005年第1期66-68,共3页Chinese Journal of Cell Biology

基　　金：国家自然科学基金资助项目(No.30270543)~~

摘　　要：　　从猪外周血分离出单个核细胞,置于EGM-2培养基中培养,通过挑选细胞集落并对之进行免疫组织化学染色和荧光染色来鉴定内皮祖细胞。结果显示猪的内皮祖细胞为长梭形或纺锤形并呈集落生长,能够吞噬已酰化低密度脂蛋(ac-LDL)并结合凝结素BS-1,同时具有内皮细胞标志CD31、flk-1和von willebrand factor(vWF)。这些结果表明能够从猪的外周血中分离培养出内皮祖细胞,为自体内皮祖细胞移植促进猪慢性心肌缺血模型血管新生的研究打下了基础。Mononuclear cells were isolated from pig peripheral blood by density-gradient centrifugation andcultured in EGM-2. After 10 to 14 days of culture, adherent cells forming colony were selected. Immunohistochem-istry and fluorescent staining was performed to identify the expressing of EC-specific antigens and functions.Results show that after 10 days of culture, the adherent cells resulted in a spindle-shaped, EC-like morphology.These cells have the ability to endocytose ac-LDL and bind BS-1 lectin. They also could be shown to have ECspecific markers such as CD31, flk-1 and vWF. These results demonstrate that endothelial progenitor cells (EPCs)can be isolated and cultured from pig peripheral blood and provide a cell source for the further investigation ofautologous EPCs transplantation contributing to promote angiogenesis in pig chronic cardiac ischemia.

关 键 词：内皮祖细胞 外周血 集落 分离培养 吞噬 血分 CD31 纺锤形 挑选 生长 

分 类 号：S852.2[农业科学—基础兽医学]