小鼠感染泡球蚴后细胞因子水平的变化  

Change of Cytokines in Mice with Echinococcus multilocularis Infection

作　　者：魏晓丽[1] 丁剑冰[1] 许晏[1] 温浩[2] 林仁勇[2] 

机构地区：[1]新疆医科大学基础医学院免疫学教研室 [2]新疆医科大学第一附属医院包虫病临床研究所,乌鲁木齐830054

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第6期361-364,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　观察人工接种泡球蚴 (EM)昆明小鼠体内 6种细胞因子水平的动态变化 ,研究泡球蚴病的免疫学机制。　方法　实验组和对照组小鼠分别腹腔接种泡球蚴及生理盐水 ,持续观察 2 60d ,收集各组血清用ELISA法检测血清中白细胞介素 2 (IL 2 )、γ干扰素 (IFN γ)、肿瘤坏死因子 α(TNF α)、IL 4、IL 5、IL 10水平。　结果　实验组 6种细胞因子水平始终高于正常对照组 ,其中辅助性T细胞 1(Th1)类细胞因子IL 2水平在感染后 80d达到峰值 ,感染 14 0d后迅速降低 ;TNF α水平在感染后 40d较对照组明显上升 ,感染 10 0d左右达到峰值 ,14 0d后迅速下降 ;IFN γ在感染 80d后达到峰值 ,14 0d后缓慢下降 ;而在 80d以前 ,辅助性T细胞 2 (Th2 )类细胞因子IL 4、IL 5、IL 10维持在较低水平 ;10 0d后 ,这 3类细胞因子明显上升 ,其中IL 4、IL 10水平在 10 0d达到峰值 ,IL 5水平在 14 0d达到峰值 ,以后维持在较高水平。　结论　Th2细胞介导的体液免疫与Th1细胞介导的细胞免疫共同参与了宿主抗棘球蚴免疫。感染早期以Th1细胞介导的细胞免疫应答为主 ,感染中晚期转化为以Th2细胞介导的体液免疫为主。Objective To observe the change of six cytokines in mice infected with Echinococcus multilocularis as part of the study on immunological mechanism in the infection. Methods Mice were infected by abdominal inoculation of echinococcus protoscoleces. The change of serum level of the cytokines IL-2、IFN-γ、TNF-α、IL-4、 IL-5 and IL-10 was determined by ELISA during the infection which lasted for 260 d. Results Compared with uninfected control, the levels of the cytokines all significantly increased in the 260 d. The level of IL-2 reached a peak after 80 d post-infection (p.i.), then decreased quickly after 140 d p.i., High level of TNF-α was detected after 40 d, compared to uninfected control, reached a peak at 100 d p.i., and decreased quickly after 140 d. The level of IFN-γ reached a peak after 80 d p.i., and decreased slowly after 140 d p.i., The levels of IL-4, IL-5 and IL-10 remained lower before 80 d, and increased sharply after 100 days. The levels of IL-4 and IL-10 reached peaks at 100 d p.i., and that of IL-5 at 140 d p.i. Conclusion The data suggest that the induction of Th2 antibody-mediated immunity (AMI) with a parallel expansion of Th1 cell-mediated inflammatory (CMI) responses are important mechanism of the host in defending against the metacestodes. Th1 CMI plays an important role at the early stage of infection, and Th2 AMI is important in the later stage of infection.

关 键 词：感染 细胞因子水平 对照组 IL-10 IL-5 IL-4 小鼠 人工接种 EM 果实 

分 类 号：R392[医药卫生—免疫学] R562.25[医药卫生—基础医学]