沙眼衣原体感染Hela229细胞诱导SOCS-1,3的表达  

Expression of SOCS-1, 3 induced by Chlamydia trachomatis-infected Hela229

作　　者：魏秀青[1] 程文[1] 严杰[1] 梅冰[1] 霍治[1] 余平[1] 

机构地区：[1]中南大学湘雅医学院免疫学教研室,长沙410078

出　　处：《中南大学学报（医学版）》2004年第6期639-642,共4页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅项目 (0 2ssy30 1 0 9)

摘　　要：目的 :探讨SOCS的表达与沙眼衣原体抑制宿主免疫功能导致持续性感染之间的关系。方法 :应用RT PCR和Westernblotting检测沙眼衣原体感染和未感染Hela2 2 9细胞SOCS 1,3mRNA水平和蛋白质水平的表达 ;免疫荧光检测反义寡核苷酸阻断SOCS 1,3表达后对沙眼衣原体增殖的影响。结果 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,阻断SOCS 1,3表达后沙眼衣原体增殖受到抑制。结论 :沙眼衣原体感染Hela2 2 9细胞后可诱导SOCS 1,3的表达 ,SOCS 1,3的表达是沙眼衣原体抑制宿主免疫功能 。Objective To explore the relationship between SOCS, immune suppression and chlamydial persistence of Chlamydia trachomatis-infected cells. Methods After being infected with Chlamydia trachomatis, RT-PCR was used to examine the expression of SOCS-1,3 mRNA and Western blotting to examine the expression of SOCS-1,3 protein in Hela229 cells. Immunofluorescence was used to examine the effect of SOCS obstruction on the multiplication of Chlamydia trachomatis. Results Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3 both on mRNA level and on protein level. The multiplicity of Chlamydia trchomatis can be suppressed after the expression of SOCS-1,3 was obstructed. Conclusion Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3, which is one of the mechanisms that Chlamydia trachomatis can suppress host immune and result in persistence.

关 键 词：SOCS 表达 沙眼衣原体感染 细胞诱导 阻断 增殖 抑制 MRNA水平 免疫荧光检测 RT—PCR 

分 类 号：R374.1[医药卫生—病原生物学]