以胶原缓释rhBMP-2复合BMSCs及珊瑚构建组织工程骨异位成骨的实验研究  

Experiment on Ectopia Osteogenesis by Tissue Engineered Bone Fabricated by Coral, Bone Mesenchymal Stem Cells and Sustainedly Released rhBMP-2 by Collagen

作　　者：程晓兵[1] 侯锐[1] 毛天球[1] 杨耀武[1] 高瞻[1] 张浚睿[1] 孙沫逸[1] 胡晓光[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科,陕西西安710032

出　　处：《口腔医学研究》2004年第6期574-576,共3页Journal of Oral Science Research

基　　金：国家"973"项目资助课题 (编号 :G19990 5 43 0 8)

摘　　要：目的 :观察以胶原缓释rhBMP - 2复合BMSCs及珊瑚构建的组织工程骨异位成骨的能力。方法 :构建 3种复合支架材料 :1)rhBMP - 2 /珊瑚 ;2 )胶原 /rhBMP - 2 /珊瑚 ;3)BMSCs/胶原 /rhBMP - 2 /珊瑚。分别植入裸鼠皮下 ,8周后观察成骨情况 ,并作比较。结果 :第 3组材料异位成骨的能力最强 ,第 2组次之 ,第 1组较弱。结论 :动物实验中胶原是rhBMP - 2适宜的缓释载体 。Objective: To evaluate ectopia osteogenesis by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen. Methods: Three scaffolds were fabricated: rhBMP-2/coral group, collagen/rhBMP-2/coral group and BMSCs/collagen/rhBMP-2/coral group. Osteogenesis was evaluated after implantation of scaffolds under skin of nude mice 8 weeks later. Results: It showed that osteogenesis in BMSCs/collagen/ rhBMP-2/coral group was more than that in collagen/rhBMP-2/coral group, and osteogenesis in rhBMP-2/coral was least. Conclusion: Collagen may be a suitable sustained release system for rhBMP-2 in animal experiment. And BMSCs may have important effect on enhancing ectopia osteogenesis.

关 键 词：Ⅰ型胶原 重组人骨形成蛋白-2 骨髓间充质干细胞 珊瑚 异位成骨 

分 类 号：R318.1[医药卫生—生物医学工程]