武汉市2003年流感监测及当前优势流行毒株的抗原性和基因特性分析  

Influenza surveillance and antigenic and genetic characterization of predominant strains in Wuhan area in 2003

作　　者：朱洪浩[1] 徐西雁 刘传楠[1] 林新明[1] 周敦金[1] 彭劲松[1] 

机构地区：[1]武汉市疾病预防控制中心,430022 [2]American Center for Disease Control and Prevention

出　　处：《中华实验和临床病毒学杂志》2004年第3期210-212,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　探讨 2 0 0 3年武汉市流感流行概况 ,分析优势流行毒株抗原性和基因特性变异特征。方法　每周在流感监测哨点医院统计流行病学资料 ,采样分离流感病毒标本 ,对其中 3株H3病毒做血凝抑制试验并对其编码HA1区基因进行序列测定和分析。结果　从 4 18份咽拭标本中分离到流感病毒 5 8株 ,其中 5 7株为H3亚型 ,1株为乙型 ;全年月分离率和门诊流感样病例数的变化有冬、夏两个高峰 ,最高峰均出现在 7月 ;新分离到的 3株H3病毒的抗原性与本年度疫苗组分株A Panama 2 0 0 7 99有较大差异 ,编码HA1区的氨基酸序列有 14个位点发生了变异 ,基因种系发生树分析也证实他们的HA1基因存在较大差异。结论　 2 0 0 3年武汉市流感活动呈双峰型增强 ,H3病毒活动显著加强 ,其抗原性和基因特性与疫苗株相比均发生了较大的变异。Objective To study influenza epidemic and analyze antigenic and genetic characterization of the predominant strains in Wuhan area in 2003 Methods Epidemiological data and specimens from influenza patients were collected from surveillance sites weekly Viruses were isolated from the specimens Three H3 isolates were chosen to do antigenic analysis by hemagglutination inhibition (HI) test and their HA1 region was sequenced Results Totally 58 influenza viruses were isolated from 418 specimens, 57 of them were identified as H3 subtype and 1 of them was B subtype; both monthly positive rate and numbers of influenza like illness had two peaks of winter and summer, the highest peak appeared in July. The 3 new H3 isolates were antigenically different from vaccine strain A/Panama/2007/99, 14 amino acid changes have been found in HA1 domain of these 3 strains compared with A/Panama/2007/99, phylogenetic analysis also confirmed the difference in HA1 domain Conclusion Influenza epidemic had two peaks in Wuhan area in 2003 The activity of H3 virus was strengthened remarkably And they are antigenically and genetically different from the vaccine strain

关 键 词：抗原性 流感监测 基因 流行 HA 流感病毒 标本 毒株 分株 血凝抑制试验 

分 类 号：R181.8[医药卫生—流行病学]