贵州白纹伊蚊对登革病毒易感性的研究  

Susceptibility of 15 collections of Aedes albopictus from Guizhou to dengue virus oral infection

作　　者：舒莉萍[1] 左丽[1] 赵星[1] 陈阿英[1] 韦隆华[1] 

机构地区：[1]贵阳医学院免疫学教研室,550004

出　　处：《中华实验和临床病毒学杂志》2004年第3期234-237,F002,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助项目 ( 3 9960 0 0 1)

摘　　要：目的　用细胞、分子生物学技术进行贵州省白纹伊蚊不同地理株对登革病毒 (DEN)易感性的研究。方法　采集贵州省 9个地 (州 )市共计 15个县 (区 )白纹伊蚊幼虫标本 ,饲养为成蚊 ;取羽化后 3～ 5日龄期的贵州不同地理株白纹伊蚊 ,用不同型别的DEN分别经口连续感染 3d ,于首次感染后的 4、7、10、14d收集感染成蚊标本 ;制备蚊悬液 ,碘化钠法提取RNA ,用DENNS1基因区通用引物经逆转录 聚合酶链反应 (RT PCR)检测DEN核酸 ;蚊悬液接种C6 36细胞进行病毒分离 ,制作细胞抗原片 ,经间接免疫荧光法检测DEN抗原 ;同时感染白纹伊蚊海南株作为对照。结果　DEN 1～ 4型国际参考株感染白纹伊蚊贵州省不同地方株 ,其感染比率分别为 12 15、12 15、8 15和 13 15。结论　白纹伊蚊贵州省不同地方株对DEN 1～ 4型国际参考株普遍易感 ,表明贵州省具备引起登革热流行的条件。Objective To study the susceptibility of Aedes albopictus to dengue virus infection Methods Aedes albopictus from 15 collections in Guizhou province were challenged orally with dengue virus 1-4 types, respectively The total RNA from mosquitos were extracted The viral NS1 gene fragment was amplified with reverse transcriptase polymerase chain reaction (RT-PCR) Dengue virus in mosquitos was isolated with C6/36 cells Then the viral antigen was detected by indirect immunofluorescence assay (IFA) Antigen and nucleic acid of dengue virus from 15 geographic strains of Aedes albopictus orally infected with dengue viruses (DEN -1,DEN -2,DEN -3 and DEN -4) were detected by IFA,RT-PCR and the virus was isolated with C6/36 cells, respectively Results The rates of Aedes albopictus orally infected with DEN-1,DEN-2,DEN-3 and DEN-4 were 12/15,12/15, 8/15 and 13/15, respectively Conclusion Different geographic strains of Aedes albopictus in Guizhou were susceptible to dengue viruses

关 键 词：白纹伊蚊 DEN 感染 易感性 登革病毒 成蚊 悬液 地理株 贵州 羽化 

分 类 号：R373[医药卫生—病原生物学] R384.1[医药卫生—基础医学]