SCID-HuIC小鼠模型的建立及在rAd5HPV16L1-E7疫苗中的应用  

The construction of SCID-Hu IC mice model and application in rAd5HPV16L1-E7 vaccine

作　　者：宋长芹[1] 李勇[2] 栾怡[3] 卞继峰[3] 赵丽[3] 赵蔚明[3] 贾继辉[3] 周亚滨[3] 齐眉[3] 于修平[3] 

机构地区：[1]山东省卫生学校检验学科,济南250002 [2]山东大学齐鲁医院检验科 [3]山东大学病原生物学研究所

出　　处：《中华实验和临床病毒学杂志》2004年第3期243-246,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助课题 ( 3 0 170 0 45 );山东省重点自然科学基金资助课题 (Z2 0 0 0C0 4)

摘　　要：目的　研究脐血移植建立SCID HuIC小鼠嵌合动物模型以及在HPV16L1 E7重组腺病毒中的应用。方法　 (1)采用尾静脉注射法给实验组注射脐血单个核细胞悬液 ,对照组注射RPMI16 4 0培养液 ;8周末检测血中人IgG抗体水平及人CD4 5、CD3、CD19;(2 )将SCID HuIC小鼠和SCID小鼠 ,分别经腹腔注射和滴鼻途径给予rAd5HPV16L1 E7重组病毒 ,于第 4周末取血检测rAd5HPV16L1 E7重组病毒特异性IgG抗体、脾细胞培养液中IFN γ的含量及T 淋巴细胞增殖。结果　 (1)实验组中人IgG抗体阳性数为 10 15 ,含量为 (0 16 7± 0 0 89) ;人CD4 5为 (9 39± 4 2 1) ,CD3为 (3 2 5± 3 99) ;CD19为 (1 6 9± 0 75 ) ,对照组未测出人IgG抗体以及CD4 5、CD3、CD19;(2 )腹腔注射组和滴鼻组病毒特异性IgG抗体均为 10 0 %阳性 ;实验组培养液中IFN γ的含量和T 淋巴细胞增殖实验病毒特异性蛋白刺激组均高于非刺激组 (P <0 0 5 ) ;对照组SCID小鼠rAd5HPV16L1 E7重组病毒特异性IgG抗体、T 淋巴细胞增殖和培养液中人IFN γ均为阴性。结论　 (1)采用人脐血移植SCID小鼠建立SCID HuIC小鼠嵌合动物模型是可行的 ;(2 )人新鲜脐血移植重建的SCID HuIC小鼠具有对rAd5HPV16L1 E7重组病毒的免疫应答能力 ,而未移植者 (对照组 )则对rAd5HPV16L1 E7重?Objective To construct human-SCID chimeric mice through implantation of mononuclear cells from human cord blood and study the immunoreaction of SCID-Hu IC mice immunized with rAd5HPV16L1-E7 vaccine Methods (1)Experiment groups were injected with the suspension of mononuclear cells from human cord blood through a tail vein; the control ones were injected with non-serum RPMI 1640 medium Eight weeks after implantation, blood was collected and human serum IgG level in the mice were tested, and human CD45, CD3 and CD19 were determined (2)SCID-Hu IC mice were divided into two groups:in group A the mice were immunized intraperitoneally with rAd5HPV16L1-E7 virus and in group B the mice were immunized through nasal drip with rAd5HPV16L1-E7 virus At the end of fourth week, the serum specific IgG antibody to rAd5HPV16L1-E7 virus, IFN-γ in culture medium of spleen lymphocyte and T-lymphocyte propagation were tested Results (1)In the experiment groups, the number of mice positive for human IgG was 10/15,the average values of CD45,CD3 and CD19 were (939±421),(325±399)and (169±075),respectivelyIn the control ones, the human IgG, CD45, CD3 and CD19 were negative (2)The results in the experiment groups showed that the IFN-γ and T-lymphocyte stimulated by HPV16 protein were higher than those in the non-stimulated group (P<005) Conclusion (1)The results indicated that the construction of human-SCID chimaera through the implantation of mononuclear cells from human cord blood into SCID mice was successfulThey also indicated that the reconstructed SCID-Hu IC mice has the ability to produce immune response against rAd5HPV16L1-E7 recombinant virus

关 键 词：HPV16 SCID小鼠 对照组 人IGG IFN-γ CD3 T-淋巴细胞 重组病毒 培养液 免疫应答能力 

分 类 号：R392[医药卫生—免疫学] R446[医药卫生—基础医学]