B→O血型改造制备通用型血过程中残留工具酶的检测  

Detection of minimal amount of residual α-galactosidase involved in conversion of blood type B to O

作　　者：王颖丽[1] 由英[1] 高红伟[1] 宫锋[1] 章扬培[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第6期546-548,557,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究发展计划 (2 0 0 2CB713 80 4);全军"十五"重点课题 (0 1Z0 2 9)

摘　　要：目的 :检测B→O血型改造制备通用型血过程中的残留工具酶—α 半乳糖苷酶。方法 :血清学反应确定α 半乳糖苷酶残酶量安全值 ,用SDS PAGE银染法和酶活性检测法测定通用型血中微量残留工具酶量。结果 :确定了α 半乳糖苷酶残酶量安全值为 10ng/ml,建立了检测微量残留α 半乳糖苷酶的方法。确定经过 6～ 7次洗涤后 ,残留酶量可达到安全水平。结论 :本研究为B→O血型改造制备的通用型血进一步的临床应用奠定了基础。Objective:To detect the minimal amount of residual α-galactosidase involved in conversion of blood type B to O.Methods:The safe amount of α-galactosidase was determined by seroconversion reaction.The minimal amount of residual α- galactosidase was detected by SDS-PAGE silver staining combined with enzyme activity assay. Results:The safe value of residual α-galactosidase was 10 ng/ml.The methods for detection of minimal α-galactosidase were established. After 6-7 times of washing the residual α-galactosidase in 'universal donor blood'reached the safe level. Conclusion:This work is the basis for further study of 'universal donor blood preparation' in clinical transfusion.

关 键 词：血型 Α-半乳糖苷酶 临床应用 工具酶 残留 血清学反应 酶活性检测 通用型 制备 微量 

分 类 号：R927[医药卫生—药学]