β-谷甾醇酯化反应过程中的反相高效液相色谱分析  

Analysis by RP-HPLC in the reaction process of β-sitosterol with succinate anhydride

作　　者：王雅琼[1] 邵强[1] 沙鸥[1] 许文林[1] 

机构地区：[1]扬州大学化学化工学院,江苏扬州225002

出　　处：《扬州大学学报（自然科学版）》2005年第1期32-34,共3页Journal of Yangzhou University：Natural Science Edition

基　　金：江苏省教育厅自然科学基金资助项目(99KJB150007);江苏省普通高等学校高新技术产业发展基金资助项目(JHB04033);江苏省"青蓝工程"跨世纪学术带头人基金资助项目

摘　　要：应用反相高效液相色谱法对β-谷甾醇与琥珀酸酐酯化反应过程中的分析测定进行了研究.色谱测定采用HypersilODS反相柱(4.6mm×150mm,5μm),流动相为色谱纯甲醇,流速为0.7mL·min-1,检测波长为210nm,恒溶剂洗脱.实验结果表明,β-谷甾醇及其酯化产物β-谷甾醇琥珀酸单酯在色谱上均有响应并达到基线分离;采用面积归一法能够快速测定β-谷甾醇酯化反应过程中各组分的含量和β-谷甾醇酯化反应的摩尔转化率.RP-HPLC was used to determine the content of β-sitosterol and β-sitosterol hemisuccinate in the reaction of β-sitosterol with succinic anhydride. Chromatographic separation was carried out using a Hypersil ODS column under isocratic conditions with methanol as the mobile phase at a flow-rate of 0.7 mL·min^(-1). The operative wavelength was set at 210 nm. The experimental results show that β-sitosterol and β-sitosterol hemisuccinate can be separated satisfactorily and the peak area normalization method can be used to determine the content of β-sitosterol and β-sitosterol hemisuccinate quantitatively. The analysis method can be used to investigate the reaction kinetics of β-sitosterol with succinic anhydride.

关 键 词：Β-谷甾醇 琥珀酸酐 酯化反应 高效液相色谱 反应动力学 

分 类 号：O657.7[理学—分析化学] TQ645.9[理学—化学]