两种体系中酶促合成(R)-苯乙氰醇的对比研究  

Comparative study on the asymmetric synthesis of (R)-mandelonitrile catalysed by (R)-oxynitrilase in two systems

作　　者：刘森林[1] 

机构地区：[1]深圳大学生命科学学院,深圳518060

出　　处：《深圳大学学报（理工版）》2005年第1期80-84,共5页Journal of Shenzhen University(Science and Engineering)

基　　金：深圳市科技局资助项目(200325)

摘　　要：在微水相和水/有机溶剂双相两种反应体系中,利用杏仁(R) 醇腈酶催化苯甲醛与HCN不对称合成(R) 苯乙氰醇,对比探讨了有机溶剂、水、pH值和温度等对两种反应体系中醇腈酶反应的影响.结果表明,上述因素对两种体系中醇腈酶促不对称合成(R) 苯乙氰醇反应均有显著影响.在常温(25℃)下,微水相中酶反应的转化率和产物光学纯度优于水/有机溶剂双相中的相应值,但水/有机溶剂双相中酶反应速度较微水相中快得多.异丙醚是两种反应体系中最好的有机溶剂,两种体系中最佳反应温度均为0～5℃.在低温(0～5℃)下,微水相和水/有机溶剂双相中平衡转化率和产物的光学纯度均高达99%以上.The asymmetric synthesis of (R)-mandelonitrile from benzaldehyde and hydrogen cyanide catalysed by (R)-oxynitrilase in organic solvent and in water/organic solvent biphase was studied. The effects of organic solvents, water, pH value and reaction temperature on the reaction in two systems were analized systematically. It was found that, at room temperature (25℃),the substrate conversion and product enantiomeric excess in organic solvent system were higher than those in water/organic solvent biphase, but the reaction rate in water/organic solvent biphase was much faster than that in organic solvent. Among all organic solvents examined, diisopropyl ether was found to be the best organic solvent for the reaction. The optimum reaction temperature was 0～5℃ in both systems. At low temperature(0～5℃),either substrate conversion or product ee% were over 99% in both systems.

关 键 词：微水相 水/有机溶剂双相 醇腈酶 (R)-苯乙氰醇 

分 类 号：Q55[生物学—生物化学] O643[理学—物理化学]