鸭茅高效丛生芽体系的建立及耐盐植株的再生  

High efficiency induction of multiple shoots from orchardgrass(Dactylis glomerata L.) and regeneration of salt-tolerant plantlets

作　　者：晁相蓉[1] 宋玲玲[2] 胡孝瑞[2] 姜素云[2] 杨爱芳[2] 

机构地区：[1]山东省卫生学校,山东济南250002 [2]山东大学生命科学学院,山东济南250100

出　　处：《山东农业科学》2005年第1期7-10,共4页Shandong Agricultural Sciences

基　　金：国家植物转基因专项(J2002-B-006);山东省优秀中青年科学家科研奖励基金(2004BS06004)资助。

摘　　要：取鸭茅无菌黄化苗生长点处的2～3mm长切段为材料,在附加不同浓度6-BA和2,4-D组合的培养基上诱导丛生芽发生。丛生芽块在继代培养中迅速增生。通过在培养基中加盐筛选,获得了耐受1 5%NaCl的耐盐小芽。这些小芽在无NaCl的培养基上恢复培养3代后转回附加1 5%NaCl的培养基上继续培养,表现出比对照小芽明显提高的耐盐性和增殖率。耐盐小芽在生根培养基上生根后移栽大田,成活率达90%以上,获得了耐盐性提高的鸭茅新种质。Multiple shoots were induced from the 2～3 mm segments of the seedling tips of aseptically orchardgrass and proliferated vigorously on media supplemented with different combinations of 2,4-D and 6-BA. Proliferating shoots were subcultured on media containing 1.5% NaCl for 3 continuous generations and salt-tolerant shoots were obtained. Salt-tolerant shoots, after being subcultured for 3 generations on NaCl-free medium, showed higher salt-tolerance and proliferating rate than the control on media supplemented with 1.5% NaCl. Salt-tolerant plantlets with vigorous root system were transplanted to pot and the surviving rate was more than 90%.

关 键 词：:鸭茅 离体培养 丛生芽 耐盐植株再生 

分 类 号：Q78[生物学—分子生物学]