转Bt基因抗虫棉卡那霉素田间鉴定技术研究  

Identification of Transgenic Bt Cotton by Using Kanamycin in Cotton Field

作　　者：朱加保[1] 杨可胜[2] 产焰坤[3] 胡积送[1] 刘方志[1] 郑曙峰[1] 徐道青[1] 江本利[1] 

机构地区：[1]安徽省农业科学院棉花研究所,安庆246003 [2]安徽省农业科学院产业处,合肥230031 [3]安徽省农业科学院土肥研究所,合肥230031

出　　处：《安徽农业大学学报》2005年第1期19-21,共3页Journal of Anhui Agricultural University

基　　金：安徽省财政厅世行 2期加灌科研项目;安徽省自然科学基金项目(03041101)共同资助

摘　　要：对转基因抗虫棉不同时期、不同叶片对卡那霉素不同浓度的反应及其与棉花抗虫性的关系进行了较系统的研究。结果表明,选取 4 000mg·L-1的卡那霉素液在棉花苗期、蕾期涂抹棉株倒二片平展叶最适宜,田间卡那霉素反应与田间棉铃虫发生实况结果吻合率高。说明棉花田间抗卡那霉素反应能够准确检测转基因抗虫棉,可以作为一种快速简便的田间检测转基因抗虫棉的方法。A simple,cheap and efficient technique for identifying transgenic Bt cotton by using kanamycin as an identification marker in the field experiment was tested.The result showed that kanamycin could be used to test whether or not a plant was resistant.In the field experiment,a writing-brush was soaked with 4 000 mg·L^(-1) kanamycin solution and scribbled on the second neonate expanded leaves at seedling and bud stage.As a result,seven days later,all of leaves of the susceptible plants showed apparent yellow patches whereas the leaves of the Bt resistant plants remained green without any symptom.Furthermore,the reaction of plants against the kanamycin corresponded to the result which was infected by bollworm in the field.

关 键 词：转基因抗虫棉 卡那霉素 鉴定 

分 类 号：S432.25[农业科学—植物病理学]