铬天青S共振光散射法测定脱氧核糖核酸  

DETERMINATION OF DEOXYRIBONUCLEIC ACID WITH CHROME AZUROL S BY RESONANCE LIGHT SCATTERING METHOD

作　　者：谭琰[1] 蔡昌群[1] 陈小明[1] 罗和安[1] 

机构地区：[1]湘潭大学化学学院,湘潭411105

出　　处：《化学分析计量》2005年第1期32-34,共3页Chemical Analysis And Meterage

基　　金：湖南省自然科学基金 (0 0JJY2 0 84);湖南省教育厅资助课题(0 3c611)

摘　　要：介绍在阳离子表面活性剂十六烷基三甲基溴化铵 (CTMAB)存在下阴离子染料铬天青S(CAS)共振光散射(RLS)法测定脱氧核糖核酸 (DNA)的方法。在pH =5 .2 7的六次甲基四铵 -盐酸缓冲溶液中 ,研究了CAS -CTMAB -yDNA体系的RLS光谱特征、影响因素和最佳反应条件。在最佳条件下 ,体系的RLS强度增加值ΔI与yDNA的浓度在 5 0～ 80 0 μg/L和 10 0 0～ 2 0 0 0 μg/L呈线性关系 ,其线性回归方程分别为ΔI=0 .4 8c+2 .5 6和ΔI=0 .14c +17.86 ,相关系数分别为 0 .9997和 0 .9991,检出限为 4 .3ng/mL。该方法简便、快速 ,应用于合成样品中yDNA的测定 ,测定结果的相对标准偏差为 2 .93%～ 4 .71% ,回收率为 97.8%～ 10 5 .2 %。The determination of deoxyribonucleic acid with chrome azurol S by resonance light scattering method in the presence of cation surfactant cetyltrimethylammonium bromide (CTMAB)was reported. In hexamethylene tetramine-HCl buffer solution(pH=5.27), the spectral characteristics of RLS, the affecting factors and the optimum conditions of the reaction were investigated. Under the optimum conditions, the enhanced RLS intensity is proportional to the concentration of yDNA in the range of 50～800 μg/L and 1000～ 2000 μg/L. The linear regression equation are Δ I=0.48c+2.56 and Δ I=0.14c+17.86 respectively, the correlation coefficient are 0.9997 and 0.9991 respectively and the detection limit is 4.3 ng/mL .This method is simple,rapid and was applied to the determination of yDNA in synthetic samples with the recoveries of 97.8 ～105.2% and the relative standard deviations of 2.93%～ 4.71% .

关 键 词：铬天青S 四铵 共振光散射 相对标准偏差 最佳反应条件 CTMAB 十六烷基三甲基溴化铵 DNA 脱氧核糖核酸 测定结果 

分 类 号：O657[理学—分析化学] R446[理学—化学]