Subcellular Localization of the S Locus F-box Protein AhSLF-S2 in Pollen and Pollen Tubes of Self-Incompatible Antirrhinum  

作　　者：Hong-YunWANG Yong-BiaoXUE 

机构地区：[1]InstituteofGeneticsandDevelopmentalBiology,theChineseAcademyofSciences,Beijing100080,China／／GraduateSchooloftheChineseAcademyofSciences,Beijing100039,China [2]InstituteofGeneticsandDevelopmentalBiology,theChineseAcademyofSciences,Beijing100080,China

出　　处：《Journal of Integrative Plant Biology》2005年第1期76-83,共8页植物学报（英文版）

基　　金：国家自然科学基金

摘　　要：The distribution of the S locus F-box (SLF) protein was examined by immunocytochemistry and Western blot techniques using an antibody against the C-terminal part of AhSLF-S2 in self-incompatible lines of Antirrhinum. Abundant gold particles were found where pollen tubes emerge in vitro. With the elongation of pollen tubes, binding sites for the antibody were found in the cytoplasm of the pollen tubes,including the peripheral part of the endoplasmic reticulum. After germination in vitro for 16 h, the product of AhSLF-S2 and possibly its allelic products could still be detectable, implying that the SLF protein has a role in the elongating process of pollen tubes. The present study provides evidence at the protein level that the SLF protein is present in pollen cytoplasm during pollen tube growth. These findings are discussed, as is their potential role in the self-incompatible response in Antirrhinum.

分 类 号：Q949.777.8[生物学—植物学] S681.9[农业科学—观赏园艺]