功能化聚乳酸微球改性聚乳酸膜片表面及其细胞相容性  

SURFACE TAILORING OF POLY(LACTIC ACID) MEMBRANE VIA SURFACE-FUNCTIONALIZED MICROSPHERES TO PROMOTE CYTOCOMPATIBILITY

作　　者：陈达[1] 计剑[1] 沈家骢[1] 

机构地区：[1]浙江大学高分子科学与工程系,杭州310027

出　　处：《高分子学报》2004年第6期826-830,共5页Acta Polymerica Sinica

基　　金：国家重点基础研究发展规划项目 (项目号G19990 5 43 0 5 )

摘　　要：以两亲三嵌段共聚物PEO PPO PEO及其氨基酸、多肽RGD衍生物作为微球制备过程中的表面稳定剂 ,低分子量的聚乳酸作为成核材料 ,采用o w乳液溶剂挥发法制备得到表面含不同氨基酸的聚乳酸微球 ,荧光标记手段证明了氨基酸存在于微球的表面 ;利用溶胀嵌入固定法将微球固定到具有生物惰性聚乳酸膜片表面 ,制备得到了具有良好稳定性的微球改性聚乳酸生物惰性膜片 ;成骨细胞相容性测试表明 ,氨基酸尤其是多肽RGD在生物惰性聚乳酸表面的引入能较大程度地提高聚乳酸生物惰性表面的细胞相容性 ,能较好地诱导细胞行为如细胞粘附、增殖等过程的发生 ,为实现对细胞行为的诱导控制提供了一种新途径 .Functiona poly(lactic acid) microspheres with core-shell structures have been prepared by using the PEO-PPO-PEO(Pluronics F68) or the ligand-tethered PEO-PPO-PEO derivatives as the surface stabilizer and poly(lactic acid) as the core material. Confocal Laser Scanning Microscopy ( CLSM) investigation indicated that the amphiphillic triblock copolymer PEO-PPO-PEO or its ligand-tethered derivatives existed on the shell of microspheres. The ligand-tethered microspheres were then embedded in cell-resistant PEO-PPO-PEO films to create cell-interactive surfaces with discrete clustered-ligand domains. The osteoblast compatibility test showed that the amino acid or peptide RGD existed on the surface of microspheres promoted osteoblast attachment and growth. This simple surface treatment method may have potentials for tissue engineering and other biomedical applications.

关 键 词：聚乳酸微球 细胞相容性 聚乳酸 RGD 诱导 氨基酸 多肽 改性 表面 功能化 

分 类 号：R318.08[医药卫生—生物医学工程]