气管内一次性注入博莱霉素诱发大鼠肺损伤的动态变化(英文)  

Consecutive change by single-dosed bleomycin-induced pulmonary fibrosis of rats

作　　者：孔璐[1] 王继峰[1] 牛建照[1] 夏志刚[1] 段斐[1] 高宝华[1] 杨美娟[1] 

机构地区：[1]北京中医药大学细胞与生化教研室,北京100029

出　　处：《中国现代医学杂志》2004年第24期14-17,21,共5页China Journal of Modern Medicine

基　　金：Supported by grants from the great project of the National Natural science foundation of China (30130220); the Special Founds of SARS and the Public Funds of Lab of Colloid and interface Science; Lnstitute of chemistry.

摘　　要：目的评估气管内一次性注入博莱霉素(bleomycin BLM)诱发大鼠肺纤维化病变最佳时间点。方法SD大鼠气管内一次性注入BLM,复制肺纤维化模型,对照组注入生理盐水,分别于7、14、28 d 1.5%戊巴比妥钠麻醉下,用肝素抗凝后注射器行腹主动脉抽血4ml,进行动脉血气分析。左肺行组织固定,右肺支气管肺泡灌洗,膜天平法进行肺表面物质(Pulmonary surfactant, PS)活性测定。灌洗后右肺液氮速冻,进行羟脯氨酸(Hydroxyproline HYP)测定。结果7 d模型组动物出现明显低氧血症,14 d逐渐好转,但仍然低于正常,28 d血氧饱和度完全恢复正常。其动态变化与体重、PS活性变化及病理组织切片的结果相一致。羟脯氨酸在14和28 d时明显增加,但两组间比较无明显差异。结论①气管内一次性注入BLM各指标变化最佳时间点在14 d。②博莱霉素通过损伤肺Ⅱ型细胞(ATⅡ),使PS系统功能异常,从而导致低氧血症的发生。Objective: To assess the most suitable time point for a single intratracheal instillation (IT) of bleomycin-induced lung fibrosis model. Methods: SD rat were treated with a single IT dose of bleomycin or control saline. On days 7, 14 and 28 following IT bleomycin or saline instillation, animals were sacrificed under overdose of 1.5% sodium pentobarbital (0.25 ml/100 g, IP) and taken blood 4ml from the abdominal aorta with heparin-treated injection syringe for arterial blood gas analysis. The left lung was fixed for routine light microscopic examination. Bronchoalveolar lavage fluid (BALF) from the right lung was tested the activity of pulmonary surfactant (PS) by the whihelmy Film Balance, then the right lung was fromzen immediately in liquid nitrogen for determination of hydroxyproline concentration. Results: Model rats had obviously hypoxemia on days 7 and improved on days 14 and complete recovery to normal on days 28. This consecutive changes was coincident with changes of body weight, the activity of PS and tissue section pathological examination. Hydroxyproline levels increased in bleomycin-treated rats on days 14 and 28. there is no statistical significant between two groups (days 14 and 28). Conclusion: ①14 days after a single intratracheal instillation of bleomycin is the most appropriate time for this model. ②PS disfunction resulted in hypoxemia after bleomycin injured alveolar type Ⅱ (AT Ⅱ).

关 键 词：博莱霉素 肺纤维化 表面活性物质 动脉血气 

分 类 号：R563[医药卫生—呼吸系统]